InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TNKS

Summary

InnateDB Protein

IDBP-6910.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TNKS

Protein Name

tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase

Synonyms

ARTD5; PARP-5a; PARP5A; PARPL; pART5; TIN1; TINF1; TNKS1;

Species

Homo sapiens

Ensembl Protein

ENSP00000311579

InnateDB Gene

IDBG-6906 (TNKS)

Protein Structure

UniProt Annotation

Function

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity. {ECO:0000269PubMed:10988299, ECO:0000269PubMed:11739745, ECO:0000269PubMed:16076287, ECO:0000269PubMed:19759537, ECO:0000269PubMed:21478859, ECO:0000269PubMed:22864114, ECO:0000269PubMed:23622245}.

Subcellular Localization

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Chromosome, centromere. Nucleus, nuclear pore complex. Chromosome, telomere {ECO:0000305}. Cytoplasm, cytoskeleton, spindle pole. Note=Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1. Localizes to spindle poles at mitosis onset via interaction with NUMA1.

Disease Associations

Tissue Specificity

Ubiquitous; highest levels in testis.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 38 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	38 [view]
Protein-Protein	38 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003950	NAD+ ADP-ribosyltransferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0006471	protein ADP-ribosylation
GO:0007052	mitotic spindle organization
GO:0007067	mitotic nuclear division
GO:0008152	metabolic process
GO:0015031	protein transport
GO:0016055	Wnt signaling pathway
GO:0018105	peptidyl-serine phosphorylation
GO:0018107	peptidyl-threonine phosphorylation
GO:0032210	regulation of telomere maintenance via telomerase
GO:0032212	positive regulation of telomere maintenance via telomerase
GO:0043392	negative regulation of DNA binding
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0051028	mRNA transport
GO:0051225	spindle assembly
GO:0070198	protein localization to chromosome, telomeric region
GO:0070212	protein poly-ADP-ribosylation
GO:0070213	protein auto-ADP-ribosylation
GO:0090263	positive regulation of canonical Wnt signaling pathway

Cellular Component

GO:0000139	Golgi membrane
GO:0000242	pericentriolar material
GO:0000775	chromosome, centromeric region
GO:0000781	chromosome, telomeric region
GO:0000784	nuclear chromosome, telomeric region
GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005643	nuclear pore
GO:0005794	Golgi apparatus
GO:0005829	cytosol
GO:0031965	nuclear membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001660 Sterile alpha motif domain
IPR002110 Ankyrin repeat
IPR011510 Sterile alpha motif, type 2
IPR012317 Poly(ADP-ribose) polymerase, catalytic domain
IPR013761 Sterile alpha motif/pointed domain
IPR020683 Ankyrin repeat-containing domain
IPR021129 Sterile alpha motif, type 1

PFAM