Bos taurus Protein: IRF3
InnateDB Protein IDBP-692919.2
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol IRF3
Protein Name Interferon regulatory factor 3
Species Bos taurus
Ensembl Protein ENSBTAP00000031815
InnateDB Gene IDBG-640634 (IRF3)
Protein Structure
UniProt Annotation
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (By similarity). {ECO:0000250}.
Subcellular Localization Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (By similarity). {ECO:0000250}.
Disease Associations
Tissue Specificity
Number of Interactions This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 83 interaction(s) predicted by orthology.
Predicted by orthology
Total 83 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0000975 regulatory region DNA binding
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0044212 transcription regulatory region DNA binding
Biological Process
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0002376 immune system process
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0009617 response to bacterium
GO:0031663 lipopolysaccharide-mediated signaling pathway
GO:0032496 response to lipopolysaccharide
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0043330 response to exogenous dsRNA
GO:0045087 innate immune response
GO:0045351 type I interferon biosynthetic process
GO:0045358 negative regulation of interferon-beta biosynthetic process
GO:0050689 negative regulation of defense response to virus by host
GO:0051607 defense response to virus
GO:0060340 positive regulation of type I interferon-mediated signaling pathway
GO:0097300 programmed necrotic cell death
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
Protein Structure and Domains
InterPro IPR001346 Interferon regulatory factor DNA-binding domain
IPR008984 SMAD/FHA domain
IPR019471 Interferon regulatory factor-3
PFAM PF00605
Post-translational Modifications
SwissProt Q4JF28
PhosphoSite PhosphoSite-
UniProt Splice Variant
Entrez Gene 516979
UniGene Bt.45332
RefSeq NP_001025016
EMBL AJ879589 BC102119 DQ103889 EU194377
GenPept AAI02120 AAZ38325 ABW74073 CAI53672