InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: SMARCA4

Summary

InnateDB Protein

IDBP-696025.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SMARCA4

Protein Name

Transcription activator BRG1

Synonyms

BAF190A; BRG1;

Species

Bos taurus

Ensembl Protein

ENSBTAP00000025598

InnateDB Gene

IDBG-643433 (SMARCA4)

Protein Structure

UniProt Annotation

Function

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-fOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self- renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000255PROSITE- ProRule:PRU00549}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 238 interaction(s) predicted by orthology.

Predicted by orthology
Total	238 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0000978	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000980	RNA polymerase II distal enhancer sequence-specific DNA binding
GO:0001105	RNA polymerase II transcription coactivator activity
GO:0002039	p53 binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003713	transcription coactivator activity
GO:0003714	transcription corepressor activity
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008094	DNA-dependent ATPase activity
GO:0008134	transcription factor binding
GO:0016787	hydrolase activity
GO:0016817	hydrolase activity, acting on acid anhydrides
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
GO:0030957	Tat protein binding
GO:0031492	nucleosomal DNA binding
GO:0042393	histone binding
GO:0047485	protein N-terminus binding
GO:0050681	androgen receptor binding
GO:0070577	lysine-acetylated histone binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0000902	cell morphogenesis
GO:0001570	vasculogenesis
GO:0001701	in utero embryonic development
GO:0001832	blastocyst growth
GO:0001835	blastocyst hatching
GO:0001889	liver development
GO:0003407	neural retina development
GO:0006200	ATP catabolic process
GO:0006337	nucleosome disassembly
GO:0006338	chromatin remodeling
GO:0006346	methylation-dependent chromatin silencing
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0007399	nervous system development
GO:0007403	glial cell fate determination
GO:0007507	heart development
GO:0010424	DNA methylation on cytosine within a CG sequence
GO:0016568	chromatin modification
GO:0019827	stem cell maintenance
GO:0022008	neurogenesis
GO:0030198	extracellular matrix organization
GO:0030216	keratinocyte differentiation
GO:0030308	negative regulation of cell growth
GO:0030900	forebrain development
GO:0030902	hindbrain development
GO:0035116	embryonic hindlimb morphogenesis
GO:0035887	aortic smooth muscle cell differentiation
GO:0043044	ATP-dependent chromatin remodeling
GO:0043388	positive regulation of DNA binding
GO:0043923	positive regulation by host of viral transcription
GO:0043966	histone H3 acetylation
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048562	embryonic organ morphogenesis
GO:0048730	epidermis morphogenesis
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0060318	definitive erythrocyte differentiation
GO:0060347	heart trabecula formation
GO:0060766	negative regulation of androgen receptor signaling pathway
GO:0070307	lens fiber cell development

Cellular Component

GO:0000790	nuclear chromatin
GO:0000792	heterochromatin
GO:0005615	extracellular space
GO:0005634	nucleus
GO:0005719	nuclear euchromatin
GO:0005726	perichromatin fibrils
GO:0005730	nucleolus
GO:0016020	membrane
GO:0016514	SWI/SNF complex
GO:0043234	protein complex
GO:0071564	npBAF complex
GO:0071565	nBAF complex

Protein Structure and Domains

PDB ID

InterPro

IPR000330 SNF2-related
IPR001487 Bromodomain
IPR001650 Helicase, C-terminal
IPR006576 BRK domain
IPR013999 HAS subgroup
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR014012 Helicase/SANT-associated, DNA binding
IPR014978 Glutamine-Leucine-Glutamine, QLQ
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00176
PF00439
PF00271
PF07533
PF07529
PF08880

PRINTS

PR00503

PIRSF

SMART

SM00297
SM00490
SM00592
SM00573
SM00487
SM00951

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt