InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ALKBH8

Summary

InnateDB Protein

IDBP-69979.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ALKBH8

Protein Name

alkB, alkylation repair homolog 8 (E. coli)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000374219

InnateDB Gene

IDBG-69973 (ALKBH8)

Protein Structure

UniProt Annotation

Function

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. {ECO:0000269PubMed:19293182, ECO:0000269PubMed:20308323}.

Subcellular Localization

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.

Disease Associations

Tissue Specificity

Widely expressed, with highest expression in spleen, followed by pancreas and lung. {ECO:0000269PubMed:17979886}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0016300	tRNA (uracil) methyltransferase activity
GO:0016491	oxidoreductase activity
GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0046872	metal ion binding

Biological Process

GO:0006974	cellular response to DNA damage stimulus
GO:0008152	metabolic process
GO:0030488	tRNA methylation
GO:0055114	oxidation-reduction process

Cellular Component

GO:0005634	nucleus
GO:0005829	cytosol

Protein Structure and Domains

PDB ID

InterPro

IPR005123 Oxoglutarate/iron-dependent dioxygenase
IPR007823 Methyltransferase-related
IPR013216 Methyltransferase type 11
IPR015095 Alkylated DNA repair protein AlkB, homologue 8, N-terminal
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF03171
PF13640
PF05148
PF08241
PF09004

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96BT7

PhosphoSite