InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DARS

Summary

InnateDB Protein

IDBP-70986.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DARS

Protein Name

aspartyl-tRNA synthetase

Synonyms

aspRS; HBSL;

Species

Homo sapiens

Ensembl Protein

ENSP00000264161

InnateDB Gene

IDBG-70984 (DARS)

Protein Structure

UniProt Annotation

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Subcellular Localization

Cytoplasm.

Disease Associations

Hypomyelination with brainstem and spinal cord involvement and leg spasticity (HBSL) [MIM:615281]: An autosomal recessive leukoencephalopathy characterized by onset in the first year of life of severe spasticity, mainly affecting the lower limbs and resulting in an inability to achieve independent ambulation. Affected individuals show delayed motor development and nystagmus; some may have mild mental retardation. Brain MRI shows hypomyelination and white matter lesions in the cerebrum, brainstem, cerebellum, and spinal cord. {ECO:0000269PubMed:23643384}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporaal cortex and the frontal polar cortex. The cerebellum, cereral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon. {ECO:0000269PubMed:23643384}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 59 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	59 [view]
Protein-Protein	57 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0004046	aminoacylase activity
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004815	aspartate-tRNA ligase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006422	aspartyl-tRNA aminoacylation
GO:0006461	protein complex assembly
GO:0010467	gene expression

Cellular Component

GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0016020	membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR002312 Aspartyl/Asparaginyl-tRNA synthetase, class IIb
IPR004364 Aminoacyl-tRNA synthetase, class II (D/K/N)
IPR004365 OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
IPR004523 Aspartyl-tRNA synthetases
IPR006195 Aminoacyl-tRNA synthetase, class II
IPR012340 Nucleic acid-binding, OB-fold

PFAM

PF00152
PF01336

PRINTS

PR01042

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P14868