InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NEIL1

Summary

InnateDB Protein

IDBP-745782.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NEIL1

Protein Name

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000457352

InnateDB Gene

IDBG-23214 (NEIL1)

Protein Structure

UniProt Annotation

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5- hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. {ECO:0000269PubMed:11904416, ECO:0000269PubMed:12200441, ECO:0000269PubMed:12509226, ECO:0000269PubMed:14522990}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269PubMed:17556049}. Nucleus {ECO:0000269PubMed:17556049}. Chromosome {ECO:0000269PubMed:17556049}. Note=During mitosis, associates with centrosomes and condensed chromatin.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:11904416}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.

Experimentally validated
Total	5 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003684	damaged DNA binding
GO:0003906	DNA-(apurinic or apyrimidinic site) lyase activity
GO:0008022	protein C-terminus binding
GO:0008270	zinc ion binding
GO:0016798	hydrolase activity, acting on glycosyl bonds
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds

Biological Process

GO:0000737	DNA catabolic process, endonucleolytic
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair
GO:0006979	response to oxidative stress
GO:0032074	negative regulation of nuclease activity

Cellular Component

GO:0005634	nucleus
GO:0005694	chromosome
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center

Protein Structure and Domains

PDB ID

InterPro

IPR010979 Ribosomal protein S13-like, H2TH
IPR012319 DNA glycosylase/AP lyase, catalytic domain
IPR015371 Endonuclease VIII-like 1, DNA binding
IPR015886 DNA glycosylase/AP lyase, H2TH DNA-binding

PFAM

PF01149
PF09292
PF06831

PRINTS

PIRSF

SMART

SM00898

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96FI4