InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ZAK

Summary

InnateDB Protein

IDBP-75237.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ZAK

Protein Name

Mitogen-activated protein kinase kinase kinase MLT

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000364361

InnateDB Gene

IDBG-75233 (ZAK)

Protein Structure

UniProt Annotation

Function

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro- apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. {ECO:0000269PubMed:10924358, ECO:0000269PubMed:11042189, ECO:0000269PubMed:11836244, ECO:0000269PubMed:15172994, ECO:0000269PubMed:15342622, ECO:0000269PubMed:15684425}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:15684425}. Nucleus {ECO:0000269PubMed:15684425}. Note=Translocates to the nucleus upon ultraviolet B irradiation.

Disease Associations

Tissue Specificity

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed. {ECO:0000269PubMed:10924358, ECO:0000269PubMed:11836244}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 49 experimentally validated interaction(s) in this database.

Experimentally validated
Total	49 [view]
Protein-Protein	48 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004709	MAP kinase kinase kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000075	cell cycle checkpoint
GO:0000077	DNA damage checkpoint
GO:0000186	activation of MAPKK activity
GO:0006468	protein phosphorylation
GO:0006950	response to stress
GO:0007010	cytoskeleton organization
GO:0007050	cell cycle arrest
GO:0007257	activation of JUN kinase activity
GO:0008219	cell death
GO:0008283	cell proliferation
GO:0009314	response to radiation
GO:0030154	cell differentiation
GO:0035556	intracellular signal transduction
GO:0043065	positive regulation of apoptotic process

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR001660 Sterile alpha motif domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011510 Sterile alpha motif, type 2
IPR013761 Sterile alpha motif/pointed domain
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR021129 Sterile alpha motif, type 1

PFAM

PF00069
PF07714
PF07647
PF00536

PRINTS

PR00109

PIRSF

SMART

SM00454
SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt