InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: EHMT2

Summary

InnateDB Protein

IDBP-79491.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

EHMT2

Protein Name

euchromatic histone-lysine N-methyltransferase 2

Synonyms

BAT8; C6orf30; G9A; GAT8; KMT1C; NG36;

Species

Homo sapiens

Ensembl Protein

ENSP00000364687

InnateDB Gene

IDBG-79489 (EHMT2)

Protein Structure

UniProt Annotation

Function

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. {ECO:0000269PubMed:11316813, ECO:0000269PubMed:18438403, ECO:0000269PubMed:20084102, ECO:0000269PubMed:20118233, ECO:0000269PubMed:22387026, ECO:0000269PubMed:8457211}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:11316813}. Chromosome {ECO:0000269PubMed:11316813}. Note=Associates with euchromatic regions. Does not associate with heterochromatin.

Disease Associations

Tissue Specificity

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. {ECO:0000269PubMed:11707778}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 117 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.

Experimentally validated
Total	117 [view]
Protein-Protein	113 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	12 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002039	p53 binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016279	protein-lysine N-methyltransferase activity
GO:0018024	histone-lysine N-methyltransferase activity
GO:0046974	histone methyltransferase activity (H3-K9 specific)
GO:0046976	histone methyltransferase activity (H3-K27 specific)
GO:0070742	C2H2 zinc finger domain binding

Biological Process

GO:0006275	regulation of DNA replication
GO:0006306	DNA methylation
GO:0016571	histone methylation
GO:0018027	peptidyl-lysine dimethylation
GO:0034968	histone lysine methylation
GO:0045087	innate immune response (InnateDB)

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005694	chromosome

Protein Structure and Domains

PDB ID

InterPro

IPR001214 SET domain
IPR002110 Ankyrin repeat
IPR003606 Pre-SET zinc-binding sub-group
IPR007728 Pre-SET domain
IPR020683 Ankyrin repeat-containing domain

PFAM

PF00856
PF00023
PF13606
PF05033
PF11929
PF12796

PRINTS

PR01415

PIRSF

SMART

SM00317
SM00248
SM00468

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt