InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ARL3

Summary

InnateDB Protein

IDBP-88148.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ARL3

Protein Name

ADP-ribosylation factor-like 3

Synonyms

ARFL3;

Species

Homo sapiens

Ensembl Protein

ENSP00000260746

InnateDB Gene

IDBG-88146 (ARL3)

Protein Structure

UniProt Annotation

Function

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. {ECO:0000269PubMed:16525022, ECO:0000269PubMed:18588884, ECO:0000269PubMed:22085962}.

Subcellular Localization

Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cell projection, cilium. Note=Detected predominantly in the photoreceptor connecting cilium. Present on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.

Disease Associations

Tissue Specificity

Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level). {ECO:0000269PubMed:12417528}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 14 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	14 [view]
Protein-Protein	14 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0004871	signal transducer activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0005525	GTP binding
GO:0008017	microtubule binding
GO:0019001	guanyl nucleotide binding
GO:0019003	GDP binding
GO:0031683	G-protein beta/gamma-subunit complex binding
GO:0046872	metal ion binding

Biological Process

GO:0000910	cytokinesis
GO:0001822	kidney development
GO:0006184	GTP catabolic process
GO:0006886	intracellular protein transport
GO:0006892	post-Golgi vesicle-mediated transport
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007224	smoothened signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0015031	protein transport
GO:0042073	intraciliary transport
GO:0042461	photoreceptor cell development
GO:0060271	cilium morphogenesis

Cellular Component

GO:0000139	Golgi membrane
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005813	centrosome
GO:0005876	spindle microtubule
GO:0005881	cytoplasmic microtubule
GO:0030496	midbody
GO:0032391	photoreceptor connecting cilium
GO:0070062	extracellular vesicular exosome
GO:0072372	primary cilium

Protein Structure and Domains

PDB ID

InterPro

IPR000795 Elongation factor, GTP-binding domain
IPR001019 Guanine nucleotide binding protein (G-protein), alpha subunit
IPR001806 Small GTPase superfamily
IPR003959 ATPase, AAA-type, core
IPR005225 Small GTP-binding protein domain
IPR006687 Small GTPase superfamily, SAR1-type
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR019009 Signal recognition particle receptor, beta subunit
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM