InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Gene: Pycard

Summary

InnateDB Gene

IDBG-210514.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pycard

Gene Name

PYD and CARD domain containing

Synonyms

9130417A21Rik; Asc; CARD5; masc; TMS-1; TNS1

Species

Mus musculus

Ensembl Gene

ENSMUSG00000030793

Encoded Proteins

IDBP-210516

PYD and CARD domain containing

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

InnateDB Annotation

Summary

PMID 21439959

Pycard is a component of the inflammasome and is required for inflammation in acute pancreatitis.

PMID 22295065

Nlrp3/Pycard inflammasome activation following human respiratory syncytial virus infection is dependent on the activation of Tlr2/Myd88/NF-kB and reactive oxygen species/potassium efflux. (Demonstrated in human)

PMID 23302887

PYCARD is an essential regulator of inflammatory responses in West Nile virus encephalitis.

PMID 24185614

Phosphorylation of the inflammasome adaptor Pycard (ASC) controls inflammasome activity through the formation of ASC specks. The NLRP3 and AIM2 inflammasomes require Syk and Mapk8 (JNK) for their full activity .

PMID 25689249

Activation of the Nlrp3 inflammasome is detrimental during leishmaniasis. Mice lacking the inflammasome components Nlrp3, Pycard, Casp1 exhibit defective Il1b and Il18 production at the infection site and are resistant to cutaneous Leishmania major infection.

PMID 26071556

Mice lacking Pycard display attenuated pulmonary hypertension and right ventricle remodelling in response to hypoxia and this is accompanied by blunted inflammasome activation.

InnateDB Annotation from Orthologs

Summary

PMID 18487086

[Homo sapiens] PYCARD protein contains CARD and Pyrin domains and is required for assembly of inflammasome.

PMID 14634131

[Homo sapiens] PYCARD prevents oligomerization of CASP1 mediated by RIPK2 by out-competing RIPK2 for binding, and thus, preventing CASP1 autoactivation. PYCARD also recruits CASP1 into PYCARD-formed cytosolic specks, separating it from RIPK2.

PMID 16585594

[Homo sapiens] PYCARD directs CASP1 away from RIPK2-mediated NF-kappaB activation, toward CASP1-mediated processing of pro-IL1B by interfering with CASP1-RIPK2 interaction.

PMID 19158675

[Homo sapiens] PYCARD binds to AIM2 to form a CASP1 and NF-kappaB activating inflammasome.

PMID 19759850

[Homo sapiens] PYCARD (ASC) splice variant protein (vASC) lacking the PGR domain regulates IL1B release and aggregates differently from intact PYCARD.

PMID 21439959

[Homo sapiens] PYCARD is a component of the inflammasome and is required for inflammation in acute pancreatitis. (Demonstrated in murine model)

PMID 22295065

[Homo sapiens] NLRP3/PYCARD inflammasome activation following human respiratory syncytial virus infection is dependent on the activation of TLR2/MYD88/NF-kB and reactive oxygen species/potassium efflux.

Entrez Gene

Summary

This gene does not have any Entrez summary - the following is the summary from its human ortholog ENSG00000103490:
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 7:127992103-127993867

Strand

Reverse strand

Band

Transcripts

ENSMUST00000033056

ENSMUSP00000033056

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 14 experimentally validated interaction(s) in this database.
They are also associated with 27 interaction(s) predicted by orthology.

Experimentally validated
Total	14 [view]
Protein-Protein	14 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	27 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002020	protease binding
GO:0005515	protein binding
GO:0016505	peptidase activator activity involved in apoptotic process
GO:0032090	Pyrin domain binding
GO:0042803	protein homodimerization activity
GO:0044325	ion channel binding

Biological Process

GO:0002218	activation of innate immune response
GO:0002230	positive regulation of defense response to virus by host
GO:0002277	myeloid dendritic cell activation involved in immune response
GO:0002588	positive regulation of antigen processing and presentation of peptide antigen via MHC class II
GO:0002821	positive regulation of adaptive immune response
GO:0006915	apoptotic process
GO:0006919	activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0006954	inflammatory response
GO:0010803	regulation of tumor necrosis factor-mediated signaling pathway
GO:0030838	positive regulation of actin filament polymerization
GO:0031647	regulation of protein stability
GO:0032088	negative regulation of NF-kappaB transcription factor activity
GO:0032314	regulation of Rac GTPase activity
GO:0032611	interleukin-1 beta production
GO:0032688	negative regulation of interferon-beta production
GO:0032729	positive regulation of interferon-gamma production
GO:0032755	positive regulation of interleukin-6 production
GO:0032760	positive regulation of tumor necrosis factor production
GO:0033209	tumor necrosis factor-mediated signaling pathway
GO:0042104	positive regulation of activated T cell proliferation
GO:0042771	intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0042981	regulation of apoptotic process
GO:0043065	positive regulation of apoptotic process
GO:0043124	negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043280	positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043281	regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0044351	macropinocytosis
GO:0045087	innate immune response (InnateDB)
GO:0046330	positive regulation of JNK cascade
GO:0050718	positive regulation of interleukin-1 beta secretion
GO:0050727	regulation of inflammatory response
GO:0050766	positive regulation of phagocytosis
GO:0050829	defense response to Gram-negative bacterium
GO:0051092	positive regulation of NF-kappaB transcription factor activity
GO:0051607	defense response to virus
GO:0070374	positive regulation of ERK1 and ERK2 cascade
GO:0071222	cellular response to lipopolysaccharide
GO:0071347	cellular response to interleukin-1
GO:0071901	negative regulation of protein serine/threonine kinase activity
GO:0090197	positive regulation of chemokine secretion
GO:0090200	positive regulation of release of cytochrome c from mitochondria
GO:2000406	positive regulation of T cell migration
GO:2000484	positive regulation of interleukin-8 secretion
GO:2000778	positive regulation of interleukin-6 secretion
GO:2001181	positive regulation of interleukin-10 secretion
GO:2001238	positive regulation of extrinsic apoptotic signaling pathway
GO:2001242	regulation of intrinsic apoptotic signaling pathway

Cellular Component

GO:0005576	extracellular region
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005783	endoplasmic reticulum
GO:0005829	cytosol
GO:0043025	neuronal cell body
GO:0043234	protein complex
GO:0072558	NLRP1 inflammasome complex
GO:0072559	NLRP3 inflammasome complex
GO:0097169	AIM2 inflammasome complex