InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DNAJA3

Summary

InnateDB Protein

IDBP-12758.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DNAJA3

Protein Name

DnaJ (Hsp40) homolog, subfamily A, member 3

Synonyms

HCA57; hTID-1; TID1;

Species

Homo sapiens

Ensembl Protein

ENSP00000262375

InnateDB Gene

IDBG-12754 (DNAJA3)

Protein Structure

UniProt Annotation

Function

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma- mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.

Subcellular Localization

Mitochondrion matrix. Cytoplasm, cytosol {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK. {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes. {ECO:0000269PubMed:9683573}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 71 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	71 [view]
Protein-Protein	70 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005083	small GTPase regulator activity
GO:0005133	interferon-gamma receptor binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008134	transcription factor binding
GO:0019901	protein kinase binding
GO:0030544	Hsp70 protein binding
GO:0030971	receptor tyrosine kinase binding
GO:0031072	heat shock protein binding
GO:0046872	metal ion binding
GO:0051059	NF-kappaB binding
GO:0051082	unfolded protein binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006264	mitochondrial DNA replication
GO:0006457	protein folding
GO:0006469	negative regulation of protein kinase activity
GO:0006915	apoptotic process
GO:0006919	activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0006924	activation-induced cell death of T cells
GO:0007005	mitochondrion organization
GO:0007264	small GTPase mediated signal transduction
GO:0007528	neuromuscular junction development
GO:0007569	cell aging
GO:0008285	negative regulation of cell proliferation
GO:0009408	response to heat
GO:0031398	positive regulation of protein ubiquitination
GO:0032088	negative regulation of NF-kappaB transcription factor activity
GO:0033077	T cell differentiation in thymus
GO:0034341	response to interferon-gamma
GO:0042102	positive regulation of T cell proliferation
GO:0043065	positive regulation of apoptotic process
GO:0043066	negative regulation of apoptotic process
GO:0043069	negative regulation of programmed cell death
GO:0043124	negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043154	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0050821	protein stabilization
GO:0060336	negative regulation of interferon-gamma-mediated signaling pathway
GO:0071340	skeletal muscle acetylcholine-gated channel clustering

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0005829	cytosol
GO:0005884	actin filament
GO:0008385	IkappaB kinase complex
GO:0019897	extrinsic component of plasma membrane
GO:0030054	cell junction
GO:0031594	neuromuscular junction
GO:0033256	I-kappaB/NF-kappaB complex
GO:0042645	mitochondrial nucleoid
GO:0043231	intracellular membrane-bounded organelle
GO:0045211	postsynaptic membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001305 Heat shock protein DnaJ, cysteine-rich domain
IPR001623 DnaJ domain
IPR002939 Chaperone DnaJ, C-terminal
IPR008971 HSP40/DnaJ peptide-binding

PFAM

PF00684
PF00226
PF01556

PRINTS

PR00625

PIRSF

SMART

SM00271

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt