InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Lig4

Summary

InnateDB Protein

IDBP-131945.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Lig4

Protein Name

ligase IV, DNA, ATP-dependent

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000093130

InnateDB Gene

IDBG-131943 (Lig4)

Protein Structure

UniProt Annotation

Function

Efficiently joins single-strand breaks in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA- dependent protein kinase complex DNA-PK to these DNA ends (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.
They are also associated with 110 interaction(s) predicted by orthology.

Experimentally validated
Total	3 [view]
Protein-Protein	0
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	110 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003909	DNA ligase activity
GO:0003910	DNA ligase (ATP) activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000012	single strand break repair
GO:0001701	in utero embryonic development
GO:0002328	pro-B cell differentiation
GO:0006266	DNA ligation
GO:0006273	lagging strand elongation
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006297	nucleotide-excision repair, DNA gap filling
GO:0006302	double-strand break repair
GO:0006303	double-strand break repair via nonhomologous end joining
GO:0006310	DNA recombination
GO:0007049	cell cycle
GO:0007417	central nervous system development
GO:0008283	cell proliferation
GO:0010165	response to X-ray
GO:0010212	response to ionizing radiation
GO:0010332	response to gamma radiation
GO:0033077	T cell differentiation in thymus
GO:0033151	V(D)J recombination
GO:0033152	immunoglobulin V(D)J recombination
GO:0033153	T cell receptor V(D)J recombination
GO:0035019	somatic stem cell maintenance
GO:0043524	negative regulation of neuron apoptotic process
GO:0045190	isotype switching
GO:0048146	positive regulation of fibroblast proliferation
GO:0050769	positive regulation of neurogenesis
GO:0051102	DNA ligation involved in DNA recombination
GO:0051103	DNA ligation involved in DNA repair
GO:0051276	chromosome organization
GO:0051301	cell division
GO:0051402	neuron apoptotic process
GO:0071285	cellular response to lithium ion
GO:0071897	DNA biosynthetic process

Cellular Component

GO:0000793	condensed chromosome
GO:0005634	nucleus
GO:0005694	chromosome
GO:0005737	cytoplasm
GO:0005886	plasma membrane
GO:0005925	focal adhesion
GO:0005958	DNA-dependent protein kinase-DNA ligase 4 complex
GO:0032807	DNA ligase IV complex
GO:0070419	nonhomologous end joining complex

Protein Structure and Domains

PDB ID

MGI:1335098

InterPro

IPR000977 DNA ligase, ATP-dependent
IPR001357 BRCT domain
IPR012308 DNA ligase, ATP-dependent, N-terminal
IPR012309 DNA ligase, ATP-dependent, C-terminal
IPR012310 DNA ligase, ATP-dependent, central
IPR012340 Nucleic acid-binding, OB-fold
IPR021536 DNA ligase IV

PFAM

PF00533
PF12738
PF04675
PF04679
PF01068
PF11411

PRINTS

PIRSF

SMART

SM00292

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8BTF7