InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RFC1

Summary

InnateDB Protein

IDBP-14030.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RFC1

Protein Name

replication factor C (activator 1) 1, 145kDa

Synonyms

A1; MHCBFB; PO-GA; RECC1; RFC; RFC140;

Species

Homo sapiens

Ensembl Protein

ENSP00000371321

InnateDB Gene

IDBG-14028 (RFC1)

Protein Structure

UniProt Annotation

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. {ECO:0000269PubMed:8999859}.Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. {ECO:0000269PubMed:8999859}.

Subcellular Localization

Nucleus.

Disease Associations

Tissue Specificity

Wide tissue distribution. Undetectable in placental tissue.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 60 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	60 [view]
Protein-Protein	59 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003689	DNA clamp loader activity
GO:0003690	double-stranded DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008047	enzyme activator activity
GO:0019904	protein domain specific binding
GO:0043565	sequence-specific DNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0000278	mitotic cell cycle
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0006200	ATP catabolic process
GO:0006260	DNA replication
GO:0006261	DNA-dependent DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006281	DNA repair
GO:0006283	transcription-coupled nucleotide-excision repair
GO:0006289	nucleotide-excision repair
GO:0006297	nucleotide-excision repair, DNA gap filling
GO:0006351	transcription, DNA-templated
GO:0007004	telomere maintenance via telomerase
GO:0032201	telomere maintenance via semi-conservative replication
GO:0043085	positive regulation of catalytic activity
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005663	DNA replication factor C complex
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0030054	cell junction
GO:0043234	protein complex
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001357 BRCT domain
IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR008921 DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal
IPR012178 DNA replication factor C, large subunit
IPR013725 DNA replication factor RFC1, C-terminal
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00533
PF12738
PF00004
PF07724
PF13304
PF08519

PRINTS

PIRSF

PIRSF036578

SMART

SM00292
SM00382

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt