InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Dgcr8

Summary

InnateDB Protein

IDBP-141486.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Dgcr8

Protein Name

DiGeorge syndrome critical region gene 8

Synonyms

D16H22S1742E; D16H22S788E; D16Wis2; Gy1; N41; Vo59c07;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000009321

InnateDB Gene

IDBG-141484 (Dgcr8)

Protein Structure

UniProt Annotation

Function

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri- miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri- miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (By similarity). Involved in the silencing of embryonic stem cells self-renewal. {ECO:0000250, ECO:0000269PubMed:17259983}.

Subcellular Localization

Nucleus {ECO:0000250}. Nucleus, nucleolus {ECO:0000250}. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. {ECO:0000269PubMed:12705904}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 23 interaction(s) predicted by orthology.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	23 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003725	double-stranded RNA binding
GO:0005515	protein binding
GO:0046872	metal ion binding

Biological Process

GO:0031053

primary miRNA processing

Cellular Component

GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0015630	microtubule cytoskeleton

Protein Structure and Domains

PDB ID

MGI:2151114

InterPro

IPR001202 WW domain
IPR014720 Double-stranded RNA-binding domain

PFAM

PF00397
PF00035

PRINTS

PIRSF

SMART

SM00456
SM00358

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9EQM6

PhosphoSite

PhosphoSite-Q9EQM6

TrEMBL

UniProt Splice Variant

Entrez Gene

94223

UniGene

RefSeq

NP_201581

MGI ID

MGI Symbol

Dgcr8

OMIM

CCDS

CCDS28018

HPRD

IMGT

EMBL

AB086857 AF201683 BC062919 BC066118

GenPept

AAG35599 AAH62919 AAH66118 BAD06468