InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ddb1

Summary

InnateDB Protein

IDBP-144532.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ddb1

Protein Name

damage specific DNA binding protein 1

Synonyms

127kDa; AA408517; p127-Ddb1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000025649

InnateDB Gene

IDBG-144530 (Ddb1)

Protein Structure

UniProt Annotation

Function

Required for DNA repair. Binds to DDB2 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. Expressed in pregnant, lactating and involuting mammary gland. {ECO:0000269PubMed:10574459}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 42 experimentally validated interaction(s) in this database.
They are also associated with 207 interaction(s) predicted by orthology.

Experimentally validated
Total	42 [view]
Protein-Protein	42 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	207 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0005515	protein binding

Biological Process

GO:0006281	DNA repair
GO:0016055	Wnt signaling pathway
GO:0035518	histone H2A monoubiquitination
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0043066	negative regulation of apoptotic process
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0070914	UV-damage excision repair
GO:1901990	regulation of mitotic cell cycle phase transition

Cellular Component

GO:0005615	extracellular space
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0031464	Cul4A-RING E3 ubiquitin ligase complex
GO:0031465	Cul4B-RING E3 ubiquitin ligase complex
GO:0070062	extracellular vesicular exosome
GO:0080008	Cul4-RING E3 ubiquitin ligase complex