InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Prf1

Summary

InnateDB Protein

IDBP-155741.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Prf1

Protein Name

perforin 1 (pore forming protein)

Synonyms

Pfn; Pfp; Prf-1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000041483

InnateDB Gene

IDBG-155739 (Prf1)

Protein Structure

UniProt Annotation

Function

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. {ECO:0000269PubMed:19446473, ECO:0000269PubMed:21037563, ECO:0000269PubMed:3261391, ECO:0000269PubMed:7526382, ECO:0000269PubMed:8164737}.

Subcellular Localization

Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen {ECO:0000250}. Note=Stored in cytoplasmic granules of cytolytic T- lymphocytes and secreted into the cleft between T-lymphocyte and target cell. May be taken up via endocytosis involving clathrin- coated vesicles and accumulate in a first time in large early endosomes (By similarity). Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in cytotoxic T-lymphocytes and natural killer cells. {ECO:0000269PubMed:2040805, ECO:0000269PubMed:2783486, ECO:0000269PubMed:3261391}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 7 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	7 [view]
Protein-Protein	2 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0022829	wide pore channel activity

Biological Process

GO:0002357	defense response to tumor cell
GO:0002418	immune response to tumor cell
GO:0019835	cytolysis
GO:0051260	protein homooligomerization
GO:0051607	defense response to virus
GO:0055085	transmembrane transport

Cellular Component

GO:0005576	extracellular region
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0016023	cytoplasmic membrane-bounded vesicle
GO:0031904	endosome lumen
GO:0044194	cytolytic granule