InnateDB Protein
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IDBP-202626.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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Spag5
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Protein Name
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sperm associated antigen 5
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Synonyms
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AI874642; D11Bhm180e; Deepest; MAP126; Mastrin; S17;
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Species
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Mus musculus
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Ensembl Protein
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ENSMUSP00000045286
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InnateDB Gene
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IDBG-202624 (Spag5)
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Protein Structure
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Function |
Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule- kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation- induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity). {ECO:0000250}.
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Subcellular Localization |
Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}. Midbody {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Note=In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity). In non-mitotic non-stressed cells, shows a microtubuli pattern. During oxidative stress, accumulates in stress granules (By similarity). {ECO:0000250}.
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Disease Associations |
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Tissue Specificity |
Detected in testis, but not in the other tissues tested. {ECO:0000269PubMed:12893248}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 10 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.
Experimentally validated |
Total |
10
[view]
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Protein-Protein |
10
[view]
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Protein-DNA |
0
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
6 [view]
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Molecular Function |
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Biological Process |
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Cellular Component |
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PDB ID |
MGI:1927470
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InterPro |
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PFAM |
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q7TME2
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PhosphoSite |
PhosphoSite-Q7TME2
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
54141
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UniGene |
Mm.24250
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RefSeq |
NP_059103
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MGI ID |
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MGI Symbol |
Spag5
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OMIM |
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CCDS |
CCDS25098
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HPRD |
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IMGT |
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EMBL |
AF420307
AL591070
AY226907
BC052672
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GenPept |
AAH52672
AAO15441
AAP46103
CAI24319
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