InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ROCK1

Summary

InnateDB Protein

IDBP-229749.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ROCK1

Protein Name

Rho-associated, coiled-coil containing protein kinase 1

Synonyms

P160ROCK; ROCK-I;

Species

Homo sapiens

Ensembl Protein

ENSP00000382697

InnateDB Gene

IDBG-1371 (ROCK1)

Protein Structure

UniProt Annotation

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. {ECO:0000269PubMed:10436159, ECO:0000269PubMed:10652353, ECO:0000269PubMed:11018042, ECO:0000269PubMed:11283607, ECO:0000269PubMed:17158456, ECO:0000269PubMed:18573880, ECO:0000269PubMed:18694941, ECO:0000269PubMed:19036714, ECO:0000269PubMed:19131646, ECO:0000269PubMed:19181962, ECO:0000269PubMed:19997641, ECO:0000269PubMed:21072057, ECO:0000269PubMed:8617235, ECO:0000269PubMed:9722579}.

Subcellular Localization

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250}. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000250}. Cell projection, lamellipodium {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Note=Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in blood platelets. {ECO:0000269PubMed:8617235}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 37 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	37 [view]
Protein-Protein	36 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0017048	Rho GTPase binding
GO:0046872	metal ion binding

Biological Process

GO:0003383	apical constriction
GO:0006468	protein phosphorylation
GO:0006915	apoptotic process
GO:0006921	cellular component disassembly involved in execution phase of apoptosis
GO:0006939	smooth muscle contraction
GO:0007159	leukocyte cell-cell adhesion
GO:0007165	signal transduction
GO:0007266	Rho protein signal transduction
GO:0007411	axon guidance
GO:0016525	negative regulation of angiogenesis
GO:0022614	membrane to membrane docking
GO:0030036	actin cytoskeleton organization
GO:0030155	regulation of cell adhesion
GO:0032060	bleb assembly
GO:0032956	regulation of actin cytoskeleton organization
GO:0032970	regulation of actin filament-based process
GO:0035556	intracellular signal transduction
GO:0043524	negative regulation of neuron apoptotic process
GO:0045616	regulation of keratinocyte differentiation
GO:0050900	leukocyte migration
GO:0050901	leukocyte tethering or rolling
GO:0051451	myoblast migration
GO:0051492	regulation of stress fiber assembly
GO:0051893	regulation of focal adhesion assembly
GO:0051894	positive regulation of focal adhesion assembly
GO:2000114	regulation of establishment of cell polarity
GO:2000145	regulation of cell motility

Cellular Component

GO:0000139	Golgi membrane
GO:0001726	ruffle
GO:0005814	centriole
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0030027	lamellipodium
GO:0032059	bleb

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR001849 Pleckstrin homology domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011072 HR1 rho-binding repeat
IPR015008 Rho binding domain
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR020684 Rho-associated protein kinase 1/2

PFAM