InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ehmt2

Summary

InnateDB Protein

IDBP-252605.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ehmt2

Protein Name

euchromatic histone lysine N-methyltransferase 2

Synonyms

Bat8; D17Ertd710e; G9a; KMT1C; NG36;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000109667

InnateDB Gene

IDBG-174783 (Ehmt2)

Protein Structure

UniProt Annotation

Function

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. {ECO:0000269PubMed:12130538, ECO:0000269PubMed:15774718, ECO:0000269PubMed:18818694, ECO:0000269PubMed:22387026}.

Subcellular Localization

Nucleus. Chromosome {ECO:0000305}. Note=Almost excluded form nucleoli. Associates with euchromatic regions. Does not associate with heterochromatin. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2 (By similarity). Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 (By similarity). Interacts with UHRF1. {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:15774718}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 27 experimentally validated interaction(s) in this database.
They are also associated with 74 interaction(s) predicted by orthology.

Experimentally validated
Total	28 [view]
Protein-Protein	25 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	74 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002039	p53 binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0018024	histone-lysine N-methyltransferase activity
GO:0046974	histone methyltransferase activity (H3-K9 specific)
GO:0046976	histone methyltransferase activity (H3-K27 specific)
GO:0070742	C2H2 zinc finger domain binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006275	regulation of DNA replication
GO:0006306	DNA methylation
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0007130	synaptonemal complex assembly
GO:0007281	germ cell development
GO:0007286	spermatid development
GO:0009566	fertilization
GO:0010424	DNA methylation on cytosine within a CG sequence
GO:0016571	histone methylation
GO:0018027	peptidyl-lysine dimethylation
GO:0034968	histone lysine methylation
GO:0035265	organ growth
GO:0045087	innate immune response (InnateDB)
GO:0051567	histone H3-K9 methylation
GO:0070734	histone H3-K27 methylation

Cellular Component

GO:0005634	nucleus
GO:0005694	chromosome

Protein Structure and Domains

PDB ID

MGI:2148922

InterPro

IPR001214 SET domain
IPR002110 Ankyrin repeat
IPR003606 Pre-SET zinc-binding sub-group
IPR007728 Pre-SET domain
IPR020683 Ankyrin repeat-containing domain

PFAM

PF00856
PF00023
PF13606
PF05033
PF11929
PF12796

PRINTS

PR01415

PIRSF

SMART

SM00317
SM00248
SM00468

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt