InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ZRANB3

Summary

InnateDB Protein

IDBP-294169.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ZRANB3

Protein Name

zinc finger, RAN-binding domain containing 3

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000383979

InnateDB Gene

IDBG-70747 (ZRANB3)

Protein Structure

UniProt Annotation

Function

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks. {ECO:0000269PubMed:21078962, ECO:0000269PubMed:22704558, ECO:0000269PubMed:22705370, ECO:0000269PubMed:22759634}.

Subcellular Localization

Nucleus. Chromosome. Note=Following DNA damage, recruited to sites of DNA damage and stalled replication forks by polyubiquitinated PCNA.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.

Experimentally validated
Total	5 [view]
Protein-Protein	4 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0004519	endonuclease activity
GO:0004520	endodeoxyribonuclease activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008270	zinc ion binding
GO:0016787	hydrolase activity
GO:0036310	annealing helicase activity
GO:0070530	K63-linked polyubiquitin binding

Biological Process

GO:0000733	DNA strand renaturation
GO:0000737	DNA catabolic process, endonucleolytic
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0009411	response to UV
GO:0031297	replication fork processing
GO:0036292	DNA rewinding
GO:0045910	negative regulation of DNA recombination
GO:0048478	replication fork protection

Cellular Component

GO:0043596

nuclear replication fork

Protein Structure and Domains

PDB ID

InterPro

IPR000330 SNF2-related
IPR001650 Helicase, C-terminal
IPR001876 Zinc finger, RanBP2-type
IPR002711 HNH endonuclease
IPR003615 HNH nuclease
IPR006935 Helicase/UvrB domain
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM