InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Herc2

Summary

InnateDB Protein

IDBP-625785.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Herc2

Protein Name

hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 2

Synonyms

D15F32S1h; D7H15F32S1; D7H15F37S1; jdf2; mKIAA0393; rjs;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000131573

InnateDB Gene

IDBG-189382 (Herc2)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that regulates ubiquitin- dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250}. Nucleus {ECO:0000250}. Note=Recruited to sites of DNA damage in response to ionising radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. {ECO:0000250}.

Disease Associations

Note=Defects in Herc2 are the cause of the runty, jerky, sterile phenotype (rjs), also known as the juvenile development and fertility phenotype (jfd2), which is characterized by reduced size, jerky gait, fertility problems including spermatocyte and oocyte abnormalities, defective maternal behavior and reduced lifespan with juvenile lethality. {ECO:0000269PubMed:10441737, ECO:0000269PubMed:9689098, ECO:0000269PubMed:9949213}.

Tissue Specificity

Highest levels are found in brain and testis with lower levels in heart, lung, liver, skeletal muscle and kidney. Little expression detected in spleen. {ECO:0000269PubMed:9689098}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.
They are also associated with 42 interaction(s) predicted by orthology.

Experimentally validated
Total	3 [view]
Protein-Protein	3 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	42 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0020037	heme binding
GO:0031625	ubiquitin protein ligase binding
GO:0032183	SUMO binding
GO:0046872	metal ion binding

Biological Process

GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0007283	spermatogenesis
GO:0016567	protein ubiquitination
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005743	mitochondrial inner membrane
GO:0005814	centriole

Protein Structure and Domains

PDB ID

MGI:103234

InterPro

IPR000408 Regulator of chromosome condensation, RCC1
IPR000433 Zinc finger, ZZ-type
IPR000569 HECT
IPR000859 CUB domain
IPR001199 Cytochrome b5-like heme/steroid binding domain
IPR004939 Anaphase-promoting complex, subunit 10/DOC domain
IPR006624 Beta-propeller repeat TECPR
IPR008979 Galactose-binding domain-like
IPR009060 UBA-like
IPR009091 Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II
IPR010606 Mib-herc2
IPR016181 Acyl-CoA N-acyltransferase
IPR021097 CPH domain

PFAM