InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: CDK4

Summary

InnateDB Protein

IDBP-686706.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CDK4

Protein Name

Cyclin-dependent kinase 4

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000009420

InnateDB Gene

IDBG-634909 (CDK4)

Protein Structure

UniProt Annotation

Function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Membrane {ECO:0000250}. Note=Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 133 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	133 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004693	cyclin-dependent protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016740	transferase activity
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0007049	cell cycle
GO:0010971	positive regulation of G2/M transition of mitotic cell cycle
GO:0016310	phosphorylation
GO:0051301	cell division
GO:0071157	negative regulation of cell cycle arrest

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR002575 Aminoglycoside phosphotransferase
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714
PF01636

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q32KY4

PhosphoSite

PhosphoSite-

TrEMBL

UniProt Splice Variant

Entrez Gene

510618

UniGene

Bt.4661

RefSeq

NP_001032683

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

BC109858

GenPept

AAI09859