Version 5.4
Bos taurus Protein: RBM4
Summary
InnateDB Protein IDBP-697203.2
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol RBM4
Protein Name RNA-binding protein 4
Synonyms
Species Bos taurus
Ensembl Protein ENSBTAP00000000541
InnateDB Gene IDBG-644561 (RBM4)
Protein Structure
UniProt Annotation
Function RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2- containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro (By similarity). {ECO:0000250}.
Subcellular Localization Nucleus {ECO:0000250}. Nucleus, nucleolus {ECO:0000250}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Note=Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress- induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation (By similarity). {ECO:0000250}.
Disease Associations
Tissue Specificity
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.
Predicted by orthology
Total 3 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0003730 mRNA 3'-UTR binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0035198 miRNA binding
GO:0036002 pre-mRNA binding
GO:0044822 poly(A) RNA binding
GO:0046872 metal ion binding
GO:0097157 pre-mRNA intronic binding
GO:0097158 pre-mRNA intronic pyrimidine-rich binding
Biological Process
GO:0000381 regulation of alternative mRNA splicing, via spliceosome
GO:0002190 cap-independent translational initiation
GO:0002192 IRES-dependent translational initiation
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0017148 negative regulation of translation
GO:0030154 cell differentiation
GO:0031047 gene silencing by RNA
GO:0032055 negative regulation of translation in response to stress
GO:0035278 negative regulation of translation involved in gene silencing by miRNA
GO:0043153 entrainment of circadian clock by photoperiod
GO:0045947 negative regulation of translational initiation
GO:0046685 response to arsenic-containing substance
GO:0046822 regulation of nucleocytoplasmic transport
GO:0051149 positive regulation of muscle cell differentiation
GO:0051403 stress-activated MAPK cascade
GO:0097167 circadian regulation of translation
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0010494 cytoplasmic stress granule
GO:0016607 nuclear speck
Protein Structure and Domains
PDB ID
InterPro IPR000504 RNA recognition motif domain
IPR001878 Zinc finger, CCHC-type
PFAM PF00076
PF00098
PRINTS
PIRSF
SMART SM00360
SM00343
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q3MHX3
PhosphoSite PhosphoSite-
TrEMBL
UniProt Splice Variant
Entrez Gene 767937
UniGene Bt.20177
RefSeq NP_001070478
HUGO
OMIM
CCDS
HPRD
IMGT
EMBL BC104570 BT030489
GenPept AAI04571 ABQ12929

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

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Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca