Version 5.4
Mus musculus Gene: Dhfr
Summary
InnateDB Gene IDBG-172010.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Dhfr
Gene Name dihydrofolate reductase
Synonyms 8430436I03Rik; AA607882; AI662710; AW555094
Species Mus musculus
Ensembl Gene ENSMUSG00000021707
Encoded Proteins
IDBP-172012
dihydrofolate reductase
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
Entrez Gene
Summary This gene does not have any Entrez summary - the following is the summary from its human ortholog ENSG00000228716:
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq, Jul 2008]
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
Gene Information
Type Protein coding
Genomic Location Chromosome 13:92354783-92389053
Strand Forward strand
Band C3
Transcripts
ENSMUST00000022218 ENSMUSP00000022218
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.
Experimentally validated
Total 1 [view]
Protein-Protein 1 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 12 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003729 mRNA binding
GO:0004146 dihydrofolate reductase activity
GO:0008144 drug binding
GO:0050661 NADP binding
GO:0051871 dihydrofolic acid binding
Biological Process
GO:0006545 glycine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0009165 nucleotide biosynthetic process
GO:0031427 response to methotrexate
GO:0035094 response to nicotine
GO:0046452 dihydrofolate metabolic process
GO:0046653 tetrahydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0055114 oxidation-reduction process
Cellular Component
GO:0005829 cytosol
Orthologs
Species
Homo sapiens
Bos taurus
Gene ID
Gene Order
ENSG00000228716
View Gene Order
ENSBTAG00000007681
Not yet available
Pathways
NETPATH
REACTOME
REACT_199047
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation pathway
REACT_249770
Cell Cycle, Mitotic pathway
REACT_189088
Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF pathway
REACT_189085
Disease pathway
REACT_204801
eNOS activation and regulation pathway
REACT_188804
Cell Cycle pathway
REACT_188937
Metabolism pathway
REACT_189089
Defective AMN causes hereditary megaloblastic anemia 1 pathway
REACT_189086
Defective GIF causes intrinsic factor deficiency pathway
REACT_189075
Defective MMAA causes methylmalonic aciduria type cblA pathway
REACT_189076
Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC pathway
REACT_189078
Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD pathway
REACT_189098
Defective CD320 causes methylmalonic aciduria pathway
REACT_189097
Defective MUT causes methylmalonic aciduria mut type pathway
REACT_189096
Defects in biotin (Btn) metabolism pathway
REACT_189095
Defective BTD causes biotidinase deficiency pathway
REACT_189093
Defective MMAB causes methylmalonic aciduria type cblB pathway
REACT_189092
Defective MTR causes methylmalonic aciduria and homocystinuria type cblG pathway
REACT_189091
Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE pathway
REACT_189090
Defective CUBN causes hereditary megaloblastic anemia 1 pathway
REACT_253002
G1/S Transition pathway
REACT_250573
Mitotic G1-G1/S phases pathway
REACT_254651
Metabolism of vitamins and cofactors pathway
REACT_198608
G1/S-Specific Transcription pathway
REACT_220137
Metabolism of folate and pterines pathway
REACT_208364
Metabolism of nitric oxide pathway
REACT_238663
Metabolism of water-soluble vitamins and cofactors pathway
REACT_206830
E2F mediated regulation of DNA replication pathway
REACT_189110
Defects in vitamin and cofactor metabolism pathway
REACT_189116
Defects in cobalamin (B12) metabolism pathway
REACT_189114
Defective TCN2 causes hereditary megaloblastic anemia pathway
REACT_189100
Defective HLCS causes multiple carboxylase deficiency pathway
KEGG
mmu00790
Folate biosynthesis pathway
mmu00670
One carbon pool by folate pathway
INOH
PID NCI
Pathway Predictions based on Human Orthology Data
NETPATH
REACTOME
REACT_683
G1/S-Specific Transcription pathway
REACT_471
E2F mediated regulation of DNA replication pathway
REACT_11167
Metabolism of folate and pterines pathway
REACT_111176
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation pathway
REACT_169312
Defective HLCS causes multiple carboxylase deficiency pathway
REACT_169313
Defective MUT causes methylmalonic aciduria mut type pathway
REACT_169316
Defective MMAA causes methylmalonic aciduria type cblA pathway
REACT_169120
Defective TCN2 causes hereditary megaloblastic anemia pathway
REACT_12389
eNOS activation and regulation pathway
REACT_21267
Mitotic G1-G1/S phases pathway
REACT_11238
Metabolism of water-soluble vitamins and cofactors pathway
REACT_169280
Defective AMN causes hereditary megaloblastic anemia 1 pathway
REACT_12508
Metabolism of nitric oxide pathway
REACT_169149
Defective MTR causes methylmalonic aciduria and homocystinuria type cblG pathway
REACT_169363
Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF pathway
REACT_169178
Defective CD320 causes methylmalonic aciduria pathway
REACT_1783
G1/S Transition pathway
REACT_169429
Defects in cobalamin (B12) metabolism pathway
REACT_169169
Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC pathway
REACT_115566
Cell Cycle pathway
REACT_169238
Defects in biotin (Btn) metabolism pathway
REACT_169403
Defective BTD causes biotidinase deficiency pathway
REACT_169415
Defective GIF causes intrinsic factor deficiency pathway
REACT_169385
Defects in vitamin and cofactor metabolism pathway
REACT_169132
Defective CUBN causes hereditary megaloblastic anemia 1 pathway
REACT_152
Cell Cycle, Mitotic pathway
REACT_169256
Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD pathway
REACT_169439
Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE pathway
REACT_111217
Metabolism pathway
REACT_169318
Defective MMAB causes methylmalonic aciduria type cblB pathway
REACT_116125
Disease pathway
REACT_11193
Metabolism of vitamins and cofactors pathway
REACT_222884
Metabolism of folate and pterines pathway
REACT_208874
Defective GIF causes intrinsic factor deficiency pathway
REACT_223924
Defects in cobalamin (B12) metabolism pathway
REACT_223300
Defective MUT causes methylmalonic aciduria mut type pathway
REACT_224358
Mitotic G1-G1/S phases pathway
REACT_218264
Defective MMAA causes methylmalonic aciduria type cblA pathway
REACT_227887
Defects in vitamin and cofactor metabolism pathway
REACT_226352
eNOS activation and regulation pathway
REACT_217107
Defects in biotin (Btn) metabolism pathway
REACT_220341
Metabolism of nitric oxide pathway
REACT_211609
G1/S Transition pathway
REACT_202103
Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD pathway
REACT_215505
Cell Cycle, Mitotic pathway
REACT_210590
Defective TCN2 causes hereditary megaloblastic anemia pathway
REACT_201998
E2F mediated regulation of DNA replication pathway
REACT_224094
Defective HLCS causes multiple carboxylase deficiency pathway
REACT_221779
Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC pathway
REACT_214934
Cell Cycle pathway
REACT_210941
Defective BTD causes biotidinase deficiency pathway
REACT_222920
Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE pathway
REACT_216256
Defective CD320 causes methylmalonic aciduria pathway
REACT_218209
Metabolism of water-soluble vitamins and cofactors pathway
REACT_209855
Metabolism of vitamins and cofactors pathway
REACT_212778
Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF pathway
REACT_219139
Metabolism pathway
REACT_217517
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation pathway
REACT_225745
Defective MMAB causes methylmalonic aciduria type cblB pathway
REACT_225533
G1/S-Specific Transcription pathway
REACT_223191
Defective CUBN causes hereditary megaloblastic anemia 1 pathway
REACT_207087
Disease pathway
REACT_212600
Defective AMN causes hereditary megaloblastic anemia 1 pathway
REACT_221291
Defective MTR causes methylmalonic aciduria and homocystinuria type cblG pathway
KEGG
hsa00670
One carbon pool by folate pathway
hsa00790
Folate biosynthesis pathway
INOH
INOH website
Folate metabolism pathway
PID NCI
e2f_pathway
E2F transcription factor network
Cross-References
SwissProt P00375
TrEMBL Q544T5
UniProt Splice Variant
Entrez Gene 13361
UniGene Mm.23695 Mm.471584
RefSeq NM_010049
OMIM
CCDS CCDS26680
HPRD
IMGT
MGI ID MGI:94890
MGI Symbol Dhfr
EMBL AK031241 AK136212 BC005796 CH466567 J00382 J00383 J00384 J00385 J00386 J00387 L26316 M10071 M10722 M10811 V00731 V00733 V00734 X56066
GenPept AAA37523 AAA37524 AAA37525 AAA37637 AAA37638 AAH05796 BAC27315 BAE22879 CAA24111 CAA24112 CAA39544 CAB43539 EDL00958
RNA Seq Atlas 13361

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca