InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DDX20

Summary

InnateDB Protein

IDBP-101099.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DDX20

Protein Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20

Synonyms

DP103; GEMIN3;

Species

Homo sapiens

Ensembl Protein

ENSP00000358716

InnateDB Gene

IDBG-101097 (DDX20)

Protein Structure

UniProt Annotation

Function

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). {ECO:0000269PubMed:18984161}.

Subcellular Localization

Cytoplasm. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

Disease Associations

Tissue Specificity

Ubiquitous.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 68 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	68 [view]
Protein-Protein	68 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0004004	ATP-dependent RNA helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0019904	protein domain specific binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0000244	spliceosomal tri-snRNP complex assembly
GO:0000387	spliceosomal snRNP assembly
GO:0006200	ATP catabolic process
GO:0006396	RNA processing
GO:0010467	gene expression
GO:0016070	RNA metabolic process
GO:0034660	ncRNA metabolic process
GO:0043065	positive regulation of apoptotic process
GO:0045892	negative regulation of transcription, DNA-templated
GO:0048477	oogenesis
GO:0050810	regulation of steroid biosynthetic process

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0016020	membrane
GO:0017053	transcriptional repressor complex
GO:0032797	SMN complex
GO:0034719	SMN-Sm protein complex
GO:0097504	Gemini of coiled bodies

Protein Structure and Domains

PDB ID

InterPro

IPR001650 Helicase, C-terminal
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR014014 RNA helicase, DEAD-box type, Q motif
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00271
PF00270

PRINTS

PIRSF

SMART

SM00490
SM00487

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9UHI6