InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RFWD2

Summary

InnateDB Protein

IDBP-104995.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RFWD2

Protein Name

ring finger and WD repeat domain 2

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000356641

InnateDB Gene

IDBG-104993 (RFWD2)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. {ECO:0000269PubMed:12466024, ECO:0000269PubMed:12615916, ECO:0000269PubMed:14739464, ECO:0000269PubMed:15103385, ECO:0000269PubMed:19805145, ECO:0000269PubMed:19837670, ECO:0000269PubMed:21625211}.

Subcellular Localization

Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.

Disease Associations

Tissue Specificity

Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. {ECO:0000269PubMed:12615916, ECO:0000269PubMed:15103385}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 96 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	96 [view]
Protein-Protein	95 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0006977	DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0016567	protein ubiquitination

Cellular Component

GO:0000139	Golgi membrane
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0005829	cytosol
GO:0005925	focal adhesion
GO:0016607	nuclear speck