Homo sapiens Protein: NEK2 | |||||||||||||||||||||||||||
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Summary | |||||||||||||||||||||||||||
InnateDB Protein | IDBP-106520.6 | ||||||||||||||||||||||||||
Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||||||||||||||||
Gene Symbol | NEK2 | ||||||||||||||||||||||||||
Protein Name | NIMA (never in mitosis gene a)-related kinase 2 | ||||||||||||||||||||||||||
Synonyms | HsPK21; NEK2A; NLK1; PPP1R111; RP67; | ||||||||||||||||||||||||||
Species | Homo sapiens | ||||||||||||||||||||||||||
Ensembl Protein | ENSP00000355965 | ||||||||||||||||||||||||||
InnateDB Gene | IDBG-106516 (NEK2) | ||||||||||||||||||||||||||
Protein Structure | |||||||||||||||||||||||||||
UniProt Annotation | |||||||||||||||||||||||||||
Function | Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S- arrested cells. Isoform 2, which is not present in the nucleolus, does not. {ECO:0000269PubMed:11742531, ECO:0000269PubMed:12857871, ECO:0000269PubMed:14978040, ECO:0000269PubMed:15161910, ECO:0000269PubMed:15358203, ECO:0000269PubMed:15388344, ECO:0000269PubMed:17283141, ECO:0000269PubMed:17621308, ECO:0000269PubMed:17626005, ECO:0000269PubMed:18086858, ECO:0000269PubMed:18297113, ECO:0000269PubMed:20034488, ECO:0000269PubMed:21076410}. | ||||||||||||||||||||||||||
Subcellular Localization | Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere {ECO:0000250}. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic.Isoform 4: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear. | ||||||||||||||||||||||||||
Disease Associations | Retinitis pigmentosa 67 (RP67) [MIM:615565]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. {ECO:0000269PubMed:24043777}. Note=The disease is caused by mutations affecting the gene represented in this entry. | ||||||||||||||||||||||||||
Tissue Specificity | Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up- regulated in various cancer cell lines, as well as primary breast tumors. {ECO:0000269PubMed:11742531, ECO:0000269PubMed:15659832}. | ||||||||||||||||||||||||||
Comments | |||||||||||||||||||||||||||
Interactions | |||||||||||||||||||||||||||
Number of Interactions |
This gene and/or its encoded proteins are associated with 75 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
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Gene Ontology | |||||||||||||||||||||||||||
Molecular Function |
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Biological Process |
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Cellular Component |
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Protein Structure and Domains | |||||||||||||||||||||||||||
PDB ID | |||||||||||||||||||||||||||
InterPro |
IPR000719
Protein kinase domain IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain IPR011009 Protein kinase-like domain IPR020635 Tyrosine-protein kinase, catalytic domain |
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PFAM |
PF00069
PF07714 |
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PRINTS |
PR00109
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PIRSF | |||||||||||||||||||||||||||
SMART |
SM00220
SM00219 |
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TIGRFAMs | |||||||||||||||||||||||||||
Post-translational Modifications | |||||||||||||||||||||||||||
Modification | |||||||||||||||||||||||||||
Cross-References | |||||||||||||||||||||||||||
SwissProt | P51955 | ||||||||||||||||||||||||||
PhosphoSite | PhosphoSite-P51955 | ||||||||||||||||||||||||||
TrEMBL | |||||||||||||||||||||||||||
UniProt Splice Variant | |||||||||||||||||||||||||||
Entrez Gene | 4751 | ||||||||||||||||||||||||||
UniGene | Hs.153704 | ||||||||||||||||||||||||||
RefSeq | NP_001191112 | ||||||||||||||||||||||||||
HUGO | HGNC:7745 | ||||||||||||||||||||||||||
OMIM | 604043 | ||||||||||||||||||||||||||
CCDS | CCDS55682 | ||||||||||||||||||||||||||
HPRD | 04953 | ||||||||||||||||||||||||||
IMGT | |||||||||||||||||||||||||||
EMBL | AC096637 AK223353 AL356310 AY045701 AY863109 BC043502 BC052807 BC065932 BT019729 U11050 Z25425 Z29066 | ||||||||||||||||||||||||||
GenPept | AAA19558 AAH43502 AAH52807 AAH65932 AAK92212 AAV38534 AAW56418 BAD97073 CAA80912 CAA82309 CAH72901 | ||||||||||||||||||||||||||