InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SPRTN

Summary

InnateDB Protein

IDBP-107428.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SPRTN

Protein Name

chromosome 1 open reading frame 124

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000295050

InnateDB Gene

IDBG-107422 (SPRTN)

Protein Structure

UniProt Annotation

Function

Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. {ECO:0000269PubMed:22681887, ECO:0000269PubMed:22894931, ECO:0000269PubMed:22902628, ECO:0000269PubMed:22987070, ECO:0000269PubMed:23042605, ECO:0000269PubMed:23042607}.

Subcellular Localization

Nucleus. Chromosome. Note=Localizes to sites of UV damage via the PIP-box. Recruited to stalled relication forks at sites of replication stress.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 91 experimentally validated interaction(s) in this database.

Experimentally validated
Total	91 [view]
Protein-Protein	90 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0043130	ubiquitin binding
GO:0046872	metal ion binding
GO:0070530	K63-linked polyubiquitin binding

Biological Process

GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0009411	response to UV
GO:0019985	translesion synthesis
GO:0031398	positive regulation of protein ubiquitination