InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: EXO1

Summary

InnateDB Protein

IDBP-107720.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

EXO1

Protein Name

exonuclease 1

Synonyms

HEX1; hExoI;

Species

Homo sapiens

Ensembl Protein

ENSP00000355506

InnateDB Gene

IDBG-107718 (EXO1)

Protein Structure

UniProt Annotation

Function

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. {ECO:0000269PubMed:10364235, ECO:0000269PubMed:10608837, ECO:0000269PubMed:11809771, ECO:0000269PubMed:11842105, ECO:0000269PubMed:12414623, ECO:0000269PubMed:12704184, ECO:0000269PubMed:14636568, ECO:0000269PubMed:14676842, ECO:0000269PubMed:15225546, ECO:0000269PubMed:15886194, ECO:0000269PubMed:16143102, ECO:0000269PubMed:9685493}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:11429708, ECO:0000269PubMed:14676842, ECO:0000269PubMed:17426132}. Note=Colocalizes with PCNA to discrete nuclear foci in S-phase.

Disease Associations

Tissue Specificity

Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. {ECO:0000269PubMed:10856833, ECO:0000269PubMed:9685493, ECO:0000269PubMed:9788596, ECO:0000269PubMed:9823303}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 36 experimentally validated interaction(s) in this database.

Experimentally validated
Total	36 [view]
Protein-Protein	30 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003824	catalytic activity
GO:0004518	nuclease activity
GO:0004523	RNA-DNA hybrid ribonuclease activity
GO:0004527	exonuclease activity
GO:0005515	protein binding
GO:0008409	5'-3' exonuclease activity
GO:0035312	5'-3' exodeoxyribonuclease activity
GO:0043566	structure-specific DNA binding
GO:0045145	single-stranded DNA 5'-3' exodeoxyribonuclease activity
GO:0046872	metal ion binding
GO:0048256	flap endonuclease activity
GO:0051908	double-stranded DNA specific 5'-3' exodeoxyribonuclease activity

Biological Process

GO:0000737	DNA catabolic process, endonucleolytic
GO:0000738	DNA catabolic process, exonucleolytic
GO:0002455	humoral immune response mediated by circulating immunoglobulin
GO:0006281	DNA repair
GO:0006298	mismatch repair
GO:0006310	DNA recombination
GO:0007126	meiotic nuclear division
GO:0016446	somatic hypermutation of immunoglobulin genes
GO:0045190	isotype switching
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

InterPro

IPR006085 XPG N-terminal
IPR006086 XPG-I domain
IPR008918 Helix-hairpin-helix motif, class 2
IPR020045 5\'-3\' exonuclease, C-terminal domain
IPR029060 PIN domain-like

PFAM

PF00752
PF00867
PF01367
PF09293

PRINTS

PIRSF

SMART

SM00485
SM00484
SM00279

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9UQ84