Version 5.4
Homo sapiens Protein: MED28
Summary
InnateDB Protein IDBP-11076.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol MED28
Protein Name mediator complex subunit 28
Synonyms
Species Homo sapiens
Ensembl Protein ENSP00000237380
InnateDB Gene IDBG-11074 (MED28)
Protein Structure
UniProt Annotation
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. {ECO:0000269PubMed:15467741}.
Subcellular Localization Nucleus {ECO:0000269PubMed:15467741}. Cytoplasm {ECO:0000269PubMed:15467741}. Membrane {ECO:0000269PubMed:15467741}; Peripheral membrane protein {ECO:0000269PubMed:15467741}. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
Disease Associations
Tissue Specificity Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. {ECO:0000269PubMed:15467741}.
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 106 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.
Experimentally validated
Total 106 [view]
Protein-Protein 105 [view]
Protein-DNA 0
Protein-RNA 1 [view]
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 3 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003779 actin binding
GO:0005515 protein binding
Biological Process
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0019827 stem cell maintenance
GO:0051151 negative regulation of smooth muscle cell differentiation
Cellular Component
GO:0005856 cytoskeleton
GO:0016020 membrane
GO:0016592 mediator complex
GO:0030864 cortical actin cytoskeleton
Protein Structure and Domains
PDB ID
InterPro IPR021640 Mediator complex, subunit Med28
PFAM PF11594
PRINTS
PIRSF
SMART
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q9H204
PhosphoSite PhosphoSite-Q9H204
TrEMBL
UniProt Splice Variant
Entrez Gene 80306
UniGene Hs.605260
RefSeq NP_079481
HUGO HGNC:24628
OMIM 610311
CCDS CCDS33963
HPRD 14381
IMGT
EMBL AF317680 AF318059 AF321617 AF358829 BC011936
GenPept AAG38612 AAH11936 AAK11563 AAK32724 AAL26906

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca