InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP8

Summary

InnateDB Protein

IDBP-11728.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP8

Protein Name

ubiquitin specific peptidase 8

Synonyms

HumORF8; SPG59; UBPY;

Species

Homo sapiens

Ensembl Protein

ENSP00000302239

InnateDB Gene

IDBG-11726 (USP8)

Protein Structure

UniProt Annotation

Function

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. {ECO:0000269PubMed:16520378, ECO:0000269PubMed:17711858, ECO:0000269PubMed:18329369, ECO:0000269PubMed:9628861}.

Subcellular Localization

Cytoplasm. Nucleus {ECO:0000250}. Endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 81 experimentally validated interaction(s) in this database.
They are also associated with 10 interaction(s) predicted by orthology.

Experimentally validated
Total	81 [view]
Protein-Protein	81 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	10 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004197	cysteine-type endopeptidase activity
GO:0004843	ubiquitin-specific protease activity
GO:0005515	protein binding
GO:0017124	SH3 domain binding
GO:0036459	ubiquitinyl hydrolase activity

Biological Process

GO:0000281	mitotic cytokinesis
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007032	endosome organization
GO:0008283	cell proliferation
GO:0016579	protein deubiquitination
GO:0070536	protein K63-linked deubiquitination
GO:0071108	protein K48-linked deubiquitination

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005769	early endosome
GO:0005829	cytosol
GO:0010008	endosome membrane
GO:0019897	extrinsic component of plasma membrane
GO:0030496	midbody

Protein Structure and Domains

PDB ID

InterPro

IPR001202 WW domain
IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR001763 Rhodanese-like domain
IPR015063 USP8 dimerisation domain
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain

PFAM

PF00397
PF00443
PF00581
PF08969

PRINTS

PIRSF

SMART

SM00456
SM00450

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P40818