InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CDC34

Summary

InnateDB Protein

IDBP-12466.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CDC34

Protein Name

cell division cycle 34 homolog (S. cerevisiae)

Synonyms

E2-CDC34; UBC3; UBCH3; UBE2R1;

Species

Homo sapiens

Ensembl Protein

ENSP00000215574

InnateDB Gene

IDBG-12464 (CDC34)

Protein Structure

UniProt Annotation

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. {ECO:0000269PubMed:10329681, ECO:0000269PubMed:10373550, ECO:0000269PubMed:10871850, ECO:0000269PubMed:11675391, ECO:0000269PubMed:12037680, ECO:0000269PubMed:15652359, ECO:0000269PubMed:17461777, ECO:0000269PubMed:17698585, ECO:0000269PubMed:19112177, ECO:0000269PubMed:19126550, ECO:0000269PubMed:19945379, ECO:0000269PubMed:20061386, ECO:0000269PubMed:20347421}.

Subcellular Localization

Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Disease Associations

Tissue Specificity

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. {ECO:0000269PubMed:10373550}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 70 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	70 [view]
Protein-Protein	69 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016881	acid-amino acid ligase activity

Biological Process

GO:0000082	G1/S transition of mitotic cell cycle
GO:0000209	protein polyubiquitination
GO:0006270	DNA replication initiation
GO:0006464	cellular protein modification process
GO:0016567	protein ubiquitination
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043525	positive regulation of neuron apoptotic process
GO:0043951	negative regulation of cAMP-mediated signaling
GO:0070848	response to growth factor
GO:0070936	protein K48-linked ubiquitination
GO:0090261	positive regulation of inclusion body assembly