InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ngly1

Summary

InnateDB Protein

IDBP-133216.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ngly1

Protein Name

N-glycanase 1

Synonyms

1110002C09Rik; Png1; PNGase;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000022310

InnateDB Gene

IDBG-133214 (Ngly1)

Protein Structure

UniProt Annotation

Function

Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. {ECO:0000269PubMed:11562482, ECO:0000269PubMed:12606569, ECO:0000269PubMed:15358861}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:11562482, ECO:0000269PubMed:12606569, ECO:0000269PubMed:16055502}.

Disease Associations

Tissue Specificity

Ubiquitously expressed with highest level in testis. {ECO:0000269PubMed:11562482, ECO:0000269PubMed:12711318}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 19 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.

Experimentally validated
Total	19 [view]
Protein-Protein	19 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	11 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000224	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity
GO:0003684	damaged DNA binding
GO:0005515	protein binding
GO:0046872	metal ion binding

Biological Process

GO:0006289	nucleotide-excision repair
GO:0006516	glycoprotein catabolic process

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:1913276

InterPro

IPR002931 Transglutaminase-like
IPR006567 PUG domain
IPR006588 Peptide N glycanase, PAW domain
IPR008979 Galactose-binding domain-like
IPR018325 Rad4/PNGase transglutaminase-like fold
IPR018997 PUB domain

PFAM

PF01841
PF04721
PF03835
PF09409

PRINTS

PIRSF

SMART

SM00460
SM00580
SM00613

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9JI78