InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Trim27

Summary

InnateDB Protein

IDBP-133353.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Trim27

Protein Name

tripartite motif-containing 27

Synonyms

AW538890; Gm19403; Rfp;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000021761

InnateDB Gene

IDBG-133351 (Trim27)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T- cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus, PML body {ECO:0000250}. Endosome {ECO:0000250}. Note=Nuclear or cytoplasmic depending on the cell type. Recruited to retromer-containing endosomes via interaction with MAGEL2 (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. {ECO:0000250, ECO:0000269PubMed:10571821}.

Disease Associations

Tissue Specificity

Expressed strongly in testis. Low levels were detected in spleen, thymus, cerebrum and cerebellum. {ECO:0000269PubMed:10571821, ECO:0000269PubMed:1437549}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 11 experimentally validated interaction(s) in this database.
They are also associated with 82 interaction(s) predicted by orthology.

Experimentally validated
Total	11 [view]
Protein-Protein	11 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	82 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0002820	negative regulation of adaptive immune response
GO:0006351	transcription, DNA-templated
GO:0006469	negative regulation of protein kinase activity
GO:0032720	negative regulation of tumor necrosis factor production
GO:0032897	negative regulation of viral transcription
GO:0034314	Arp2/3 complex-mediated actin nucleation
GO:0042147	retrograde transport, endosome to Golgi
GO:0045087	innate immune response
GO:0045814	negative regulation of gene expression, epigenetic
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0070206	protein trimerization
GO:0070534	protein K63-linked ubiquitination
GO:0072643	interferon-gamma secretion
GO:0090281	negative regulation of calcium ion import
GO:1900041	negative regulation of interleukin-2 secretion
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005768	endosome
GO:0016605	PML body
GO:0030904	retromer complex
GO:0031965	nuclear membrane

Protein Structure and Domains

PDB ID

MGI:97904

InterPro

IPR000315 Zinc finger, B-box
IPR001841 Zinc finger, RING-type
IPR001870 B30.2/SPRY domain
IPR003877 SPla/RYanodine receptor SPRY
IPR003879 Butyrophylin-like
IPR006574 SPRY-associated
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR018355 SPla/RYanodine receptor subgroup
IPR018957 Zinc finger, C3HC4 RING-type
IPR020457 Zinc finger, B-box, chordata

PFAM

PF00643
PF13639
PF14634
PF00622
PF13765
PF00097

PRINTS

PR01407
PR01406

PIRSF

SMART

SM00336
SM00184
SM00449
SM00589

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt