InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Uba1

Summary

InnateDB Protein

IDBP-134808.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Uba1

Protein Name

ubiquitin-like modifier activating enzyme 1

Synonyms

A1S9; Sbx; Ube-1; Ube1x;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000001989

InnateDB Gene

IDBG-134806 (Uba1)

Protein Structure

UniProt Annotation

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system. Activates ubiquitin by first adenylating its C- terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (By similarity). {ECO:0000250}.Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin- E1 thioester and free AMP.

Subcellular Localization

Cytoplasm {ECO:0000250}. Mitochondrion {ECO:0000250}. Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. In testis, expressed in A spermatogonia and spermatids but at very low levels in pachytene spermatocytes. {ECO:0000269PubMed:8948595}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 18 experimentally validated interaction(s) in this database.
They are also associated with 76 interaction(s) predicted by orthology.

Experimentally validated
Total	18 [view]
Protein-Protein	18 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	76 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008641	small protein activating enzyme activity
GO:0016874	ligase activity
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006464	cellular protein modification process
GO:0006974	cellular response to DNA damage stimulus
GO:0016567	protein ubiquitination

Cellular Component

GO:0000792	heterochromatin
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005765	lysosomal membrane
GO:0010008	endosome membrane
GO:0030057	desmosome
GO:0030867	rough endoplasmic reticulum membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:98890

InterPro

IPR000011 Ubiquitin/SUMO-activating enzyme E1
IPR000127 Ubiquitin-activating enzyme repeat
IPR000594 UBA/THIF-type NAD/FAD binding fold
IPR009036 Molybdenum cofactor biosynthesis, MoeB
IPR018075 Ubiquitin-activating enzyme, E1
IPR018965 Ubiquitin-activating enzyme e1, C-terminal
IPR019572 Ubiquitin-activating enzyme

PFAM

PF02134
PF00899
PF09358
PF10585

PRINTS

PR01849

PIRSF

SMART

SM00985

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt