InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rad21

Summary

InnateDB Protein

IDBP-140000.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rad21

Protein Name

RAD21 homolog (S. pombe)

Synonyms

mKIAA0078; SCC1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000022927

InnateDB Gene

IDBG-139998 (Rad21)

Protein Structure

UniProt Annotation

Function

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. Plays a role in apoptosis, via its cleavage by caspase- 3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C- terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway (By similarity). The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. {ECO:0000250}.

Subcellular Localization

Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Once cleaved by caspase-3, the C- terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis (By similarity). Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed with highest levels in testis, brain, kidney, heart and thymus. Lowest levels in skeletal muscle. {ECO:0000269PubMed:8812457}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 29 experimentally validated interaction(s) in this database.
They are also associated with 218 interaction(s) predicted by orthology.

Experimentally validated
Total	29 [view]
Protein-Protein	28 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	218 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0006281	DNA repair
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006915	apoptotic process
GO:0007059	chromosome segregation
GO:0007067	mitotic nuclear division
GO:0071168	protein localization to chromatin

Cellular Component

GO:0000228	nuclear chromosome
GO:0000775	chromosome, centromeric region
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0008278	cohesin complex
GO:0016020	membrane
GO:0034991	nuclear meiotic cohesin complex

Protein Structure and Domains

PDB ID

MGI:108016

InterPro

IPR003768 Prokaryotic chromosome segregation/condensation protein ScpA
IPR006909 Rad21/Rec8-like protein, C-terminal, eukaryotic
IPR006910 Rad21/Rec8-like protein, N-terminal

PFAM

PF02616
PF04824
PF04825

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q61550

PhosphoSite