InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Slc3a2

Summary

InnateDB Protein

IDBP-140477.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Slc3a2

Protein Name

solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2

Synonyms

4F2; 4F2HC; AI314110; Cd98; Ly-10; Ly-m10; Ly10; Mdu1; Mgp-2hc; NACAE;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000010239

InnateDB Gene

IDBG-140475 (Slc3a2)

Protein Structure

UniProt Annotation

Function

Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. {ECO:0000269PubMed:10391915, ECO:0000269PubMed:11011012, ECO:0000269PubMed:9915839}.

Subcellular Localization

Apical cell membrane; Single-pass type II membrane protein. Melanosome {ECO:0000250}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (By similarity). Localized to the plasma membrane when associated with SLC7A5 or SLC7A8 (By similarity). Localized to the placental apical membrane (By similarity). Located selectively at cell-cell adhesion sites. Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Observed in all adult tissues tested with strongest expression in kidney, small intestine, spleen, thymus and liver. Moderate expression in brain, stomach, heart, testis, lung, skin, pancreas and skeletal muscle. Expressed on the surface of epithelial cells of most embryonic tissues including epidermis, choroid plexus in the brain, retina, as well as intestinal, renal, and thymic epithelium. In brain expressed on capillary endothelia in cerebral cortex. {ECO:0000269PubMed:10574970, ECO:0000269PubMed:2928113, ECO:0000269PubMed:9915839}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.
They are also associated with 31 interaction(s) predicted by orthology.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	31 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003725	double-stranded RNA binding
GO:0003824	catalytic activity
GO:0005515	protein binding
GO:0015175	neutral amino acid transmembrane transporter activity
GO:0043169	cation binding

Biological Process

GO:0005975	carbohydrate metabolic process
GO:0015827	tryptophan transport
GO:0043330	response to exogenous dsRNA
GO:0060356	leucine import

Cellular Component

GO:0016021	integral component of membrane
GO:0016324	apical plasma membrane
GO:0042470	melanosome

Protein Structure and Domains

PDB ID

MGI:96955

InterPro

IPR006047 Glycosyl hydrolase, family 13, catalytic domain
IPR006589 Glycosyl hydrolase, family 13, subfamily, catalytic domain
IPR017853 Glycoside hydrolase, superfamily

PFAM

PF00128

PRINTS

PIRSF

SMART

SM00642

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P10852