InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CIITA

Summary

InnateDB Protein

IDBP-14385.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CIITA

Protein Name

class II, major histocompatibility complex, transactivator

Synonyms

C2TA; CIITAIV; MHC2TA; NLRA;

Species

Homo sapiens

Ensembl Protein

ENSP00000316328

InnateDB Gene

IDBG-14377 (CIITA)

Protein Structure

UniProt Annotation

Function

Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). {ECO:0000269PubMed:11172716, ECO:0000269PubMed:16600381, ECO:0000269PubMed:17493635, ECO:0000269PubMed:24036077}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:23007646}. Nucleus, PML body {ECO:0000269PubMed:23007646}. Note=Recruited to PML body by PML.

Disease Associations

Bare lymphocyte syndrome 2 (BLS2) [MIM:209920]: A severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon- gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. {ECO:0000269PubMed:10501838, ECO:0000269PubMed:11466404, ECO:0000269PubMed:11862382, ECO:0000269PubMed:7749984, ECO:0000269PubMed:8402893}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 69 experimentally validated interaction(s) in this database.
They are also associated with 19 interaction(s) predicted by orthology.

Experimentally validated
Total	69 [view]
Protein-Protein	28 [view]
Protein-DNA	41 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	19 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003713	transcription coactivator activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0005525	GTP binding
GO:0008022	protein C-terminus binding
GO:0016301	kinase activity
GO:0016746	transferase activity, transferring acyl groups
GO:0032403	protein complex binding
GO:0033613	activating transcription factor binding
GO:0044212	transcription regulatory region DNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0006955	immune response
GO:0016310	phosphorylation
GO:0019221	cytokine-mediated signaling pathway
GO:0032966	negative regulation of collagen biosynthetic process
GO:0034341	response to interferon-gamma
GO:0045345	positive regulation of MHC class I biosynthetic process
GO:0045348	positive regulation of MHC class II biosynthetic process
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0046677	response to antibiotic
GO:0060333	interferon-gamma-mediated signaling pathway

Cellular Component

GO:0005654	nucleoplasm
GO:0016605	PML body

Protein Structure and Domains

PDB ID

InterPro

IPR003590 Leucine-rich repeat, ribonuclease inhibitor subtype
IPR007111 NACHT nucleoside triphosphatase
IPR008095 MHC class II transactivator
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PRINTS

PR01719

PIRSF

SMART

SM00368

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P33076