InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Mylip

Summary

InnateDB Protein

IDBP-148024.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Mylip

Protein Name

myosin regulatory light chain interacting protein

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000047403

InnateDB Gene

IDBG-148022 (Mylip)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. {ECO:0000269PubMed:19520913, ECO:0000269PubMed:20427281}.

Subcellular Localization

Cytoplasm {ECO:0000305}. Cell membrane {ECO:0000250}; Peripheral membrane protein.

Disease Associations

Tissue Specificity

Expressed in liver, spleen, intestine and adrenals. {ECO:0000269PubMed:19520913}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	11 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008092	cytoskeletal protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity

Biological Process

GO:0006928	cellular component movement
GO:0007399	nervous system development
GO:0010989	negative regulation of low-density lipoprotein particle clearance
GO:0016567	protein ubiquitination
GO:0031648	protein destabilization
GO:0032803	regulation of low-density lipoprotein particle receptor catabolic process
GO:0042632	cholesterol homeostasis
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0045732	positive regulation of protein catabolic process
GO:0055098	response to low-density lipoprotein particle stimulus

Cellular Component

GO:0005622	intracellular
GO:0005737	cytoplasm
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0019898	extrinsic component of membrane

Protein Structure and Domains

PDB ID

MGI:2388271

InterPro

IPR000299 FERM domain
IPR000798 Ezrin/radixin/moesin like
IPR001841 Zinc finger, RING-type
IPR018979 FERM, N-terminal
IPR019748 FERM central domain
IPR019749 Band 4.1 domain
IPR019750 Band 4.1 family
IPR029071 Ubiquitin-related domain

PFAM

PF13639
PF14634
PF09379
PF00373

PRINTS

PR00661
PR00935

PIRSF

SMART

SM00184
SM00295

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt