Mus musculus Protein: Rad23b | |||||||||||||||||||||||
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Summary | |||||||||||||||||||||||
InnateDB Protein | IDBP-148613.6 | ||||||||||||||||||||||
Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||||||||||||
Gene Symbol | Rad23b | ||||||||||||||||||||||
Protein Name | RAD23b homolog (S. cerevisiae) | ||||||||||||||||||||||
Synonyms | 0610007D13Rik; AV001138; HR23B; mHR23B; p58; | ||||||||||||||||||||||
Species | Mus musculus | ||||||||||||||||||||||
Ensembl Protein | ENSMUSP00000030134 | ||||||||||||||||||||||
InnateDB Gene | IDBG-148611 (Rad23b) | ||||||||||||||||||||||
Protein Structure | |||||||||||||||||||||||
UniProt Annotation | |||||||||||||||||||||||
Function | Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. {ECO:0000269PubMed:12815074, ECO:0000269PubMed:15336624, ECO:0000269PubMed:16709668}.Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with Cetn2 appears to stabilize Xpc. May protect Xpc from proteasomal degradation (By similarity). {ECO:0000250}.The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, Xpa, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the Xpc:Rad23b dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. Xpc:Rad23b contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. Xpc:Rad23bB induces a bend in DNA upon binding. Xpc:Rad23b stimulates the activity of DNA glycosylases Tdg and Smug1 (By similarity). {ECO:0000250}. | ||||||||||||||||||||||
Subcellular Localization | Nucleus {ECO:0000269PubMed:15336624}. Cytoplasm {ECO:0000269PubMed:15336624}. | ||||||||||||||||||||||
Disease Associations | |||||||||||||||||||||||
Tissue Specificity | |||||||||||||||||||||||
Comments | |||||||||||||||||||||||
Interactions | |||||||||||||||||||||||
Number of Interactions |
This gene and/or its encoded proteins are associated with 24 experimentally validated interaction(s) in this database.
They are also associated with 63 interaction(s) predicted by orthology.
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Gene Ontology | |||||||||||||||||||||||
Molecular Function |
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Biological Process |
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Cellular Component |
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Protein Structure and Domains | |||||||||||||||||||||||
PDB ID | MGI:105128 | ||||||||||||||||||||||
InterPro |
IPR000449
Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal IPR000626 Ubiquitin-like IPR004806 UV excision repair protein Rad23 IPR006636 Heat shock chaperonin-binding IPR009060 UBA-like IPR015360 XPC-binding domain IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote IPR022617 Rad60/SUMO-like domain IPR029071 Ubiquitin-related domain |
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PFAM |
PF00627
PF00240 PF14560 PF09280 PF11976 |
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PRINTS |
PR01839
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PIRSF | |||||||||||||||||||||||
SMART |
SM00213
SM00727 SM00165 |
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TIGRFAMs | |||||||||||||||||||||||
Post-translational Modifications | |||||||||||||||||||||||
Modification | |||||||||||||||||||||||
Cross-References | |||||||||||||||||||||||
SwissProt | P54728 | ||||||||||||||||||||||
PhosphoSite | PhosphoSite-P54728 | ||||||||||||||||||||||
TrEMBL | Q3UQN3 | ||||||||||||||||||||||
UniProt Splice Variant | |||||||||||||||||||||||
Entrez Gene | 19359 | ||||||||||||||||||||||
UniGene | Mm.457176 | ||||||||||||||||||||||
RefSeq | NP_033037 | ||||||||||||||||||||||
MGI ID | 2F4O | ||||||||||||||||||||||
MGI Symbol | Rad23b | ||||||||||||||||||||||
OMIM | |||||||||||||||||||||||
CCDS | CCDS18194 | ||||||||||||||||||||||
HPRD | |||||||||||||||||||||||
IMGT | |||||||||||||||||||||||
EMBL | AK142280 AK150089 AK160880 AK160890 AK160973 AL683890 BC027747 X92411 | ||||||||||||||||||||||
GenPept | AAH27747 BAE25006 BAE29298 BAE36067 BAE36071 BAE36124 CAA63146 CAM13976 | ||||||||||||||||||||||