InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Syne2

Summary

InnateDB Protein

IDBP-149686.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Syne2

Protein Name

synaptic nuclear envelope 2

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000047697

InnateDB Gene

IDBG-149682 (Syne2)

Protein Structure

UniProt Annotation

Function

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein- containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Specifically, Syne2 and Sun2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for centrosome migration to the apical cell surface during early ciliogenesis. {ECO:0000269PubMed:18477613, ECO:0000269PubMed:19596800, ECO:0000269PubMed:20724637}.

Subcellular Localization

Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane {ECO:0000250}; Single-pass type IV membrane protein {ECO:0000250}. Cell membrane; Single-pass membrane protein. Cytoplasm, cytoskeleton. Mitochondrion {ECO:0000250}. Nucleus, nucleoplasm {ECO:0000250}. Note=Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane- associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter solubles isoforms can be found in the cytoplasm and within the nucleus (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

C-terminal isoforms are highly expressed in the brain, hert and skeletal muscle. Isoform 1 (Nesprin-2 Giant) is most prevalent in the brain, skin, kidney and skeletal muscle. {ECO:0000269PubMed:18477613}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 18 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	18 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003779	actin binding
GO:0005515	protein binding

Biological Process

GO:0006998	nuclear envelope organization
GO:0007097	nuclear migration
GO:0007163	establishment or maintenance of cell polarity
GO:0010761	fibroblast migration
GO:0030335	positive regulation of cell migration
GO:0031022	nuclear migration along microfilament
GO:0034504	protein localization to nucleus
GO:0051642	centrosome localization
GO:0090286	cytoskeletal anchoring at nuclear membrane

Cellular Component

GO:0005634	nucleus
GO:0005635	nuclear envelope
GO:0005640	nuclear outer membrane
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005856	cytoskeleton
GO:0005925	focal adhesion
GO:0016021	integral component of membrane
GO:0016235	aggresome
GO:0016529	sarcoplasmic reticulum
GO:0030016	myofibril
GO:0030018	Z disc
GO:0031258	lamellipodium membrane
GO:0031527	filopodium membrane
GO:0031965	nuclear membrane
GO:0031981	nuclear lumen
GO:0033017	sarcoplasmic reticulum membrane
GO:0034993	SUN-KASH complex
GO:0045111	intermediate filament cytoskeleton

Protein Structure and Domains

PDB ID

MGI:2449316

InterPro

IPR001715 Calponin homology domain
IPR002017 Spectrin repeat
IPR012315 KASH domain
IPR018159 Spectrin/alpha-actinin
IPR022613 Calmodulin-regulated spectrin-associated protein, CH domain

PFAM

PF00307
PF00435
PF10541
PF11971

PRINTS

PIRSF

SMART

SM00033
SM00150

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q6ZWQ0