InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Gfi1b

Summary

InnateDB Protein

IDBP-152548.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Gfi1b

Protein Name

growth factor independent 1B

Synonyms

Gfi-1B;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000028156

InnateDB Gene

IDBG-152546 (Gfi1b)

Protein Structure

UniProt Annotation

Function

Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma- satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. {ECO:0000269PubMed:11696536, ECO:0000269PubMed:12351384, ECO:0000269PubMed:12594258, ECO:0000269PubMed:15718298, ECO:0000269PubMed:17707228, ECO:0000269PubMed:9566867}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12594258}. Note=Localized at foci of pericentric heterochromatin.

Disease Associations

Tissue Specificity

Expressed in bone marrow and in spleen. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Expressed in thymocytes. {ECO:0000269PubMed:12351384, ECO:0000269PubMed:12594258, ECO:0000269PubMed:17095621, ECO:0000269PubMed:9566867}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 15 experimentally validated interaction(s) in this database.
They are also associated with 75 interaction(s) predicted by orthology.

Experimentally validated
Total	15 [view]
Protein-Protein	14 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	75 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001085	RNA polymerase II transcription factor binding
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0046872	metal ion binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0007275	multicellular organismal development
GO:0030854	positive regulation of granulocyte differentiation
GO:0045646	regulation of erythrocyte differentiation
GO:0051572	negative regulation of histone H3-K4 methylation
GO:0051574	positive regulation of histone H3-K9 methylation