InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Puf60

Summary

InnateDB Protein

IDBP-152637.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Puf60

Protein Name

poly-U binding splicing factor 60

Synonyms

2410104I19Rik; 2810454F19Rik; SIAHBP1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000002599

InnateDB Gene

IDBG-152633 (Puf60)

Protein Structure

UniProt Annotation

Function

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'- splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus. Note=Colocalizes partially with TROVE2. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.
They are also associated with 81 interaction(s) predicted by orthology.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	81 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0042802	identical protein binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006397	mRNA processing
GO:0006915	apoptotic process
GO:0008380	RNA splicing