InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP5

Summary

InnateDB Protein

IDBP-15269.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP5

Protein Name

ubiquitin specific peptidase 5 (isopeptidase T)

Synonyms

ISOT;

Species

Homo sapiens

Ensembl Protein

ENSP00000229268

InnateDB Gene

IDBG-15267 (USP5)

Protein Structure

UniProt Annotation

Function

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. {ECO:0000269PubMed:19098288}.

Subcellular Localization

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 76 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	76 [view]
Protein-Protein	73 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004197	cysteine-type endopeptidase activity
GO:0004221	ubiquitin thiolesterase activity
GO:0005515	protein binding
GO:0008242	omega peptidase activity
GO:0008270	zinc ion binding
GO:0036459	ubiquitinyl hydrolase activity

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0071108	protein K48-linked deubiquitination

Cellular Component

GO:0005764

lysosome

Protein Structure and Domains

PDB ID

InterPro

IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR001607 Zinc finger, UBP-type
IPR009060 UBA-like
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR016652 Ubiquitinyl hydrolase
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain

PFAM

PF00627
PF00443
PF02148

PRINTS

PIRSF

PIRSF016308

SMART

SM00290
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt