InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Nfil3

Summary

InnateDB Protein

IDBP-152925.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Nfil3

Protein Name

nuclear factor, interleukin 3, regulated

Synonyms

AV225605; E4BP4;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000065363

InnateDB Gene

IDBG-152923 (Nfil3)

Protein Structure

UniProt Annotation

Function

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites (By similarity). Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells (By similarity). Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. {ECO:0000250, ECO:0000269PubMed:11316793, ECO:0000269PubMed:15087429, ECO:0000269PubMed:17182630, ECO:0000269PubMed:9122243}.

Subcellular Localization

Nucleus {ECO:0000255PROSITE- ProRule:PRU00978, ECO:0000269PubMed:11316793}.

Disease Associations

Tissue Specificity

Expressed in suprachiasmatic nucleus and liver (at protein level). Expressed in suprachiasmatic nucleus, hippocampus, gyrus dentatus, piriform cortex, internal granular layer of olfactory bulb, dorsomedial hypothalamic nucleus, pontine nuclei, granular layer of cerebellum, liver and calvariae osteoblasts. {ECO:0000269PubMed:11316793, ECO:0000269PubMed:12743120}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.
They are also associated with 15 interaction(s) predicted by orthology.

Experimentally validated
Total	8 [view]
Protein-Protein	2 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	15 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0000979	RNA polymerase II core promoter sequence-specific DNA binding
GO:0001078	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0005515	protein binding
GO:0043565	sequence-specific DNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0007623	circadian rhythm
GO:0010628	positive regulation of gene expression
GO:0045087	innate immune response (InnateDB)
GO:0071353	cellular response to interleukin-4

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

MGI:109495

InterPro

IPR004827 Basic-leucine zipper domain
IPR010533 Vertebrate interleukin-3 regulated transcription factor
IPR016743 Transcription factor, basic leucine zipper, E4BP4

PFAM

PF00170
PF07716
PF06529

PRINTS

PIRSF

PIRSF019029

SMART

SM00338

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt