InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: H2afy

Summary

InnateDB Protein

IDBP-156984.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

H2afy

Protein Name

H2A histone family, member Y

Synonyms

H2AF12M; macroH2A1; mH2a1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000016081

InnateDB Gene

IDBG-156982 (H2afy)

Protein Structure

UniProt Annotation

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. {ECO:0000269PubMed:16107708}.

Subcellular Localization

Nucleus. Chromosome. Note=Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.

Disease Associations

Tissue Specificity

Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla. {ECO:0000269PubMed:10471737, ECO:0000269PubMed:11262398}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.
They are also associated with 30 interaction(s) predicted by orthology.

Experimentally validated
Total	13 [view]
Protein-Protein	13 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	30 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000182	rDNA binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0005515	protein binding
GO:0010385	double-stranded methylated DNA binding
GO:0019901	protein kinase binding
GO:0030291	protein serine/threonine kinase inhibitor activity
GO:0031490	chromatin DNA binding
GO:0044212	transcription regulatory region DNA binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006334	nucleosome assembly
GO:0007549	dosage compensation
GO:0016568	chromatin modification
GO:0033128	negative regulation of histone phosphorylation
GO:0045814	negative regulation of gene expression, epigenetic
GO:0071169	establishment of protein localization to chromatin
GO:0071901	negative regulation of protein serine/threonine kinase activity
GO:1901837	negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
GO:1902750	negative regulation of cell cycle G2/M phase transition

Cellular Component

GO:0000786	nucleosome
GO:0000790	nuclear chromatin
GO:0000793	condensed chromosome
GO:0001740	Barr body
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005813	centrosome
GO:0035098	ESC/E(Z) complex
GO:0070062	extracellular vesicular exosome