Version 5.4
Mus musculus Protein: Asah2
Summary
InnateDB Protein IDBP-158402.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Asah2
Protein Name N-acylsphingosine amidohydrolase 2
Synonyms AI585898;
Species Mus musculus
Ensembl Protein ENSMUSP00000093830
InnateDB Gene IDBG-158400 (Asah2)
Protein Structure
UniProt Annotation
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. {ECO:0000269PubMed:14557071, ECO:0000269PubMed:16126722, ECO:0000269PubMed:16380386}.
Subcellular Localization Cell membrane {ECO:0000269PubMed:11591392, ECO:0000269PubMed:12921776}; Single-pass type II membrane protein {ECO:0000269PubMed:11591392, ECO:0000269PubMed:12921776}. Note=The neutral ceramidase soluble form is a secreted protein.
Disease Associations
Tissue Specificity Widely expressed. Strongly expressed in liver and kidney. Highly expressed in the small intestine along the brush border. Localizes in the apical membranes of proximal and distal tubules, collecting ducts of kidney, endosome-like organelles of hepatocytes, and in the epithelia of the jejunum and ileum. {ECO:0000269PubMed:10753931, ECO:0000269PubMed:16380386}.
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 1 [view]
Protein-Protein 1 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0017040 ceramidase activity
Biological Process
GO:0006665 sphingolipid metabolic process
GO:0006915 apoptotic process
GO:0008283 cell proliferation
GO:0010033 response to organic substance
Cellular Component
GO:0005739 mitochondrion
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
Protein Structure and Domains
PDB ID MGI:1859310
InterPro IPR006823 Neutral/alkaline nonlysosomal ceramidase
PFAM PF04734
PRINTS
PIRSF
SMART
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q9JHE3
PhosphoSite PhosphoSite-Q9JHE3
TrEMBL B9EHG7
UniProt Splice Variant
Entrez Gene 54447
UniGene Mm.404449
RefSeq NP_061300
MGI ID
MGI Symbol Asah2
OMIM
CCDS CCDS29749
HPRD
IMGT
EMBL AB037111 AB037181 AK047692 AK080951 AK136189 AK166100 BC022604 BC137898 BC145016 CH466534
GenPept AAH22604 AAI37899 AAI45017 BAA94545 BAA94546 BAC33126 BAC38089 BAE22865 BAE38571 EDL41717

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca