InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Atg3

Summary

InnateDB Protein

IDBP-162823.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Atg3

Protein Name

autophagy-related 3 (yeast)

Synonyms

2610016C12Rik; APG3; Apg3l; Atg3l; PC3-96;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000023343

InnateDB Gene

IDBG-162821 (Atg3)

Protein Structure

UniProt Annotation

Function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. {ECO:0000269PubMed:11825910, ECO:0000269PubMed:18768753, ECO:0000269PubMed:20723759, ECO:0000269PubMed:24089205}.

Subcellular Localization

Cytoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 7 experimentally validated interaction(s) in this database.
They are also associated with 24 interaction(s) predicted by orthology.

Experimentally validated
Total	7 [view]
Protein-Protein	7 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	24 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0019776	Atg8 ligase activity
GO:0019777	Atg12 ligase activity
GO:0019787	small conjugating protein ligase activity
GO:0019899	enzyme binding

Biological Process

GO:0000045	autophagic vacuole assembly
GO:0006464	cellular protein modification process
GO:0006914	autophagy
GO:0015031	protein transport
GO:0016567	protein ubiquitination
GO:0043653	mitochondrial fragmentation involved in apoptotic process
GO:1902017	regulation of cilium assembly