InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Cdc34

Summary

InnateDB Protein

IDBP-170433.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Cdc34

Protein Name

cell division cycle 34 homolog (S. cerevisiae)

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000020550

InnateDB Gene

IDBG-170431 (Cdc34)

Protein Structure

UniProt Annotation

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 49 interaction(s) predicted by orthology.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	49 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016881	acid-amino acid ligase activity

Biological Process

GO:0000209	protein polyubiquitination
GO:0006513	protein monoubiquitination
GO:0007049	cell cycle
GO:0016567	protein ubiquitination
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043525	positive regulation of neuron apoptotic process
GO:0043951	negative regulation of cAMP-mediated signaling
GO:0070848	response to growth factor
GO:0070936	protein K48-linked ubiquitination
GO:0090261	positive regulation of inclusion body assembly

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:102657

InterPro

IPR000608 Ubiquitin-conjugating enzyme, E2
IPR016135 Ubiquitin-conjugating enzyme/RWD-like

PFAM

PF00179

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8CFI2

PhosphoSite

PhosphoSite-Q8CFI2

TrEMBL

UniProt Splice Variant

Entrez Gene

216150

UniGene

RefSeq

NP_808281

MGI ID

MGI Symbol

Cdc34

OMIM

CCDS

CCDS23983

HPRD

IMGT

EMBL

BC039160 BC094502

GenPept

AAH39160 AAH94502