InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Med25

Summary

InnateDB Protein

IDBP-180155.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Med25

Protein Name

mediator of RNA polymerase II transcription, subunit 25 homolog (yeast)

Synonyms

2610034E13Rik; 2610529E18Rik; ESTM2; X83308;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000003049

InnateDB Gene

IDBG-180153 (Med25)

Protein Structure

UniProt Annotation

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
They are also associated with 43 interaction(s) predicted by orthology.

Experimentally validated
Total	5 [view]
Protein-Protein	5 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	43 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008134	transcription factor binding
GO:0042974	retinoic acid receptor binding
GO:0046965	retinoid X receptor binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0035563	positive regulation of chromatin binding
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048147	negative regulation of fibroblast proliferation
GO:0071158	positive regulation of cell cycle arrest
GO:2001178	positive regulation of mediator complex assembly

Cellular Component

GO:0005575	cellular_component
GO:0005654	nucleoplasm

Protein Structure and Domains

PDB ID

MGI:1922863

InterPro

IPR002035 von Willebrand factor, type A
IPR021394 Mediator complex, subunit Med25, PTOV activation and synapsin 2
IPR021397 Mediator complex, subunit Med25, synapsin 1
IPR021406 Mediator complex, subunit Med25, NR box
IPR021419 Mediator complex, subunit Med25, von Willebrand factor type A

PFAM

PF00092
PF11232
PF11235
PF11244
PF11265

PRINTS

PIRSF

SMART

SM00327

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8VCB2